CPR1 Antibody

CPR1 in Plant-Nematode Interactions

CPR1 (Cysteine Protease 1) is a secreted effector protein produced by the soybean cyst nematode (Heterodera glycines) that facilitates infection by suppressing plant immune responses . Key findings include:

Functional Role of CPR1

• Immune Suppression: CPR1 inhibits effector-triggered immunity (ETI) in plants by cleaving soybean mitochondrial protein GmBCAT1, a branched-chain amino acid aminotransferase .

• Protease Activity: Catalytic cysteine residue (C323) is critical for its function. Mutating C323 to serine (C323S) abolishes protease activity and immune suppression .

• Virulence Enhancement: Transgenic soybean roots expressing CPR1 showed increased susceptibility to nematode infection (33% reduction in penetration frequency when CPR1 is silenced) .

Potential for Antibody Development

While no direct studies on CPR1-specific antibodies were identified, its conserved structure and critical role in virulence make it a candidate for antibody-based targeting in agricultural biotechnology.

CRP-1/CRIP1 Antibody (ab211631)

CRP-1 (Cysteine-Rich Protein 1), also known as CRIP1, is a human intracellular zinc transport protein. The antibody ab211631 (Abcam) targets its C-terminal region .

CTRP1/C1qTNF1 Antibody (MAB10445)

CTRP1 (C1q and TNF-Related Protein 1) is a metabolic regulator. The monoclonal antibody MAB10445 (R&D Systems) detects human CTRP1 .

Plant Immune Regulation

In Arabidopsis, the F-box protein CPR1 (CONSTITUTIVE EXPRESSER OF PR GENES 1) regulates NLR immune receptors like SNC1 and RPS2 by promoting their degradation via the ubiquitin-proteasome system . Key insights:

• Autoimmunity Control: cpr1 mutants exhibit autoimmunity due to NLR overaccumulation, suppressed by SNC1 knockout .

• Ubiquitination: CPR1 interacts with SNC1 and RPS2 in vivo, enhancing their ubiquitination and degradation .

Therapeutic Antibody Development

While CPR1-targeted antibodies are not explicitly documented, advances in antibody engineering (e.g., Chugai Pharmabody Research’s high-throughput platforms ) highlight methodologies applicable to developing CPR1-specific antibodies.

Challenges and Opportunities

• Nomenclature Clarity: Distinguishing CPR1 (nematode protease), CPR1 (plant F-box protein), and human CRP-1/CTRP1 is critical for targeted research.

• Antibody Applications: Existing antibodies against CRP-1/CTRP1 (e.g., ab211631, MAB10445) serve as models for future CPR1-specific antibody development.