creb3l2 Antibody

Definition and Function

The CREB3L2 antibody is a polyclonal immunoglobulin G (IgG) antibody designed to detect the CREB3L2 protein, a non-canonical unfolded protein response (UPR) transcription factor. CREB3L2 belongs to the OASIS family, which regulates stress-induced gene expression in the endoplasmic reticulum (ER) and Golgi apparatus . Its N-terminal fragment functions as a transcription factor, while the C-terminal fragment is secreted and implicated in immune modulation .

Western Blotting

• Detects full-length (FL-CREB3L2) and cleaved N-terminal (N-CREB3L2) fragments in cell lysates .

• Recommended dilution: 1:500–1:2000 .

Immunofluorescence

• Visualizes CREB3L2 localization in fixed cells (e.g., HepG2 hepatocytes) .

• Suggested dilution: 1:50–1:500 .

ELISA

• Quantifies CREB3L2 levels in conditioned media or lysates .

Role in Tumor Immune Evasion

• CREB3L2 promotes triple-negative breast cancer (TNBC) progression by suppressing CD8+ T cell activation via the Hedgehog pathway . Antibody-based studies confirm its overexpression in TNBC and correlation with reduced CD8+ T cell infiltration .

Plasma Cell Survival

• CREB3L2 regulates long-lived plasma cell (LLPC) survival by controlling CD28/SLP-76 and CD93 expression . Antibody-mediated detection is critical for validating these findings .

Therapeutic Potential

• Targeting CREB3L2 with neutralizing antibodies or inhibitors (e.g., S1P/S2P inhibitors) may enhance cancer immunotherapy efficacy .