CRK11 Antibody
Description
CRK Antibodies
CRK (v-crk avian sarcoma virus CT10 oncogene homolog) is a proto-oncogene encoding an adaptor protein involved in signaling pathways regulating cell adhesion, migration, and apoptosis. Key antibodies targeting CRK include:
Functional Insights:
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CRK-II mediates MAPK8 activation and cell motility via Rac-dependent pathways .
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Dysregulation of CRK/CRKL is implicated in cancer progression and developmental disorders .
Kelch-Like Protein 11 (KLHL11) Antibodies
KLHL11 antibodies are distinct from CRK antibodies but share a similar naming convention. These antibodies are biomarkers for paraneoplastic neurological syndromes:
| Antibody Name | Assay Type | Clinical Utility | Tumor Associations |
|---|---|---|---|
| KLHL11-IgG | Cell Binding Assay | Diagnoses encephalitis, cerebellar ataxia | Seminoma, teratoma |
Key Findings:
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KLHL11 antibodies are detected in 44% of patients with paraneoplastic syndromes, often concurrent with testicular or ovarian tumors .
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Validation involves GFP-tagged KLHL11 transfected HEK293 cells and immunofluorescence .
Antibody Validation Methodologies
Recent studies emphasize rigorous validation to address reproducibility crises in antibody research:
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KO Validation: CRISPR/Cas9 knockout cell lines (e.g., HEK293) confirm antibody specificity .
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Multi-Application Testing: High-quality antibodies (e.g., GTX634482 for C9ORF72) are validated across immunoblot, IP, and IF .
Potential Nomenclature Confusions
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CRK vs. CRKL: CRK-like protein (CRKL) is a distinct adaptor molecule but shares functional overlap with CRK .
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CRK vs. KLHL11: KLHL11 is unrelated to CRK but may cause confusion due to similar alphanumeric naming .
Research Gaps and Recommendations
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No studies in the provided sources directly address a "CRK11 Antibody."
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Future work should clarify whether "CRK11" refers to a novel target, a typographical error (e.g., CRKL or KLHL11), or a plant-specific CRK (e.g., Arabidopsis CRK28) .
