CRK13 Antibody

CRK13 Overview

CRK13 (Gene ID: AT4G23210) belongs to the cysteine-rich receptor-like kinase (CRK) subfamily, characterized by a DUF26 domain containing a C-X8-C-X2-C motif . It is implicated in:

• Pathogen defense: Overexpression induces hypersensitive response (HR)-associated cell death and resistance to Pseudomonas syringae .

• Salicylic acid (SA) signaling: CRK13-mediated resistance is SA-dependent, with upregulated expression of defense genes (PR1, PR5, ICS1) and SA accumulation .

• Transcriptional regulation: Expression peaks within 4 hours of avirulent P. syringae infection .

CRK13 Antibody Development and Applications

While no peer-reviewed studies explicitly detail CRK13 antibody production, methodologies from analogous research suggest:

• Recombinant protein expression: CRK13 antibodies are likely generated using epitopes from its extracellular DUF26 domain or intracellular kinase domain.

• Functional assays: Antibodies would enable:

  • Immunoblotting to quantify CRK13 expression levels .

  • Immunoprecipitation to study CRK13’s interaction with pathogen-responsive complexes (e.g., FLS2) .

  • Localization studies via immunofluorescence .

Pathogen Resistance Mechanisms

Regulatory Interactions

• FLS2 association: CRK13 may interact with the FLS2 receptor complex to amplify pattern-triggered immunity (PTI) signals .

• LecRK-VI.2 pathway: CRK13 is upregulated by LecRK-VI.2, a positive PTI regulator, though its overexpression alone does not enhance bacterial resistance .

Technical Challenges and Limitations

• Lethality: High CRK13 overexpression under constitutive promoters (e.g., 35S) causes developmental defects, necessitating inducible systems (e.g., dexamethasone) .

• Antibody specificity: Cross-reactivity with other DUF26-containing CRKs (e.g., CRK4, CRK36) requires stringent validation .

Future Directions

• CRK13 antibody validation: Develop monoclonal antibodies to distinguish CRK13 from homologous CRKs.

• Mechanistic studies: Elucidate CRK13’s role in SA biosynthesis and its crosstalk with jasmonic acid/ethylene pathways.

• Agricultural applications: Engineer CRK13 overexpression in crops for broad-spectrum disease resistance .