CRK31 Antibody

Overview of Crk Family Proteins

Crk proteins are adaptor molecules critical for intracellular signaling, with roles in immune regulation, cytoskeletal dynamics, and stress responses. Key isoforms include:

Antibody Development for Crk Proteins

Several Crk-targeting antibodies are commercially available, but none are specific to CRK31:

Research Gaps

• No CRK31 identification: The Arabidopsis CRK gene family (e.g., CRK1–CRK45) includes members like CRK28 and CRK36, but CRK31 is not listed in phylogenetic analyses or T-DNA insertion studies .

• Antibody specificity: Existing antibodies target conserved domains in Crk isoforms (e.g., SH2/SH3 domains) but lack validation for CRK31 .

Potential Explanations for Absence

• Terminology mismatch: CRK31 may refer to a hypothetical or newly discovered protein not yet characterized in public databases.

• Species specificity: CRK31 could be a plant-specific isoform (e.g., in Phaseolus or Glycine max) not covered in the provided sources .

Recommendations for Future Research

1. Genomic databases: Query UniProt (ID: P46109 for CrkL) or TAIR (for Arabidopsis CRKs) for CRK31 homology.

2. Antibody validation: If CRK31 is a novel target, develop custom antibodies using immunogens from conserved regions (e.g., DUF26 domains in plants ).

3. Functional studies: Investigate CRK31’s role in immune or stress pathways using CRISPR/Cas9 knockout models.