CS Human

Citrate Synthase Human Recombinant
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Description

Chondroitin Sulfate (CS) in Osteoarthritis Treatment

Chondroitin sulfate, a glycosaminoglycan, has been studied for its effects on human cartilage and inflammatory markers in osteoarthritis (OA):

Key Findings from In Vitro/In Vivo Studies

CS CompoundEffect on Catabolic PathwaysDosageModel TypeSource
CS1Increased IL-6, MMP-1; reduced collagen type II expression200–1,000 µg/mLHuman chondrocytes
CS2Inhibited IL-6, PGE₂ production1,000 µg/mLHuman chondrocytes
CS3Limited IL-1β-induced collagen type II inhibition200 µg/mLHuman chondrocytes

Cigarette Smoke (CS) Exposure Models

CS-induced models are widely used to study chronic obstructive pulmonary disease (COPD) and airway inflammation:

Natural Compounds Mitigating CS-Induced Damage

CompoundSource PlantModel SystemMechanismEffective DoseStudy Type
BaicalinScutellaria baicalensisCS-exposed mice/HBE cellsAnti-inflammatory, antioxidant40–160 mg/kgIn vivo
SilibininSilybum marianumCS/LPS-induced miceSuppressed TGF-β1/Smad2/3 signaling10–20 mg/kgIn vivo
IcariinEpimediumCSE-exposed BEAS-2B cellsReversed glucocorticoid resistance20–80 µMIn vitro

These compounds show promise in reducing oxidative stress and fibrosis in CS-associated COPD .

Conditioned Stimuli (CS) in Human Fear Extinction

In psychology, "CS" refers to conditioned stimuli used in fear conditioning studies:

Key Results from Fear Extinction Research

  • Compound Extinction: Combining extinguished (CSA) and non-extinguished (CSB) stimuli during extinction training reduced spontaneous fear recovery in humans (P < 0.001) .

  • SCR (Skin Conductance Response): Post-extinction, the deepened extinction group showed significantly lower SCRs to CSA compared to controls .

Mechanism: Compound extinction converts CS into a safety signal by promoting elemental processing of multimodal cues (visual + auditory) .

Cesium (Cs) in Medical Applications

Although cesium (Cs) is distinct from "CS," its isotopes and compounds have human applications:

Medical Uses of Cesium

  • Radiation Dosimetry: Cs salts in thermoluminescent dosimeters quantify radiation exposure via photomultiplier-detected light pulses .

  • Catalysis: Cs enhances catalysts for acrylic acid and phthalic anhydride synthesis, critical in polymer industries .

Carbon Disulfide (CS₂) Toxicity and Regulation

While not directly "CS Human," carbon disulfide exposure impacts occupational health:

EPA Risk Assessment Framework

  • DCAP (Derived Chemical Assessment Program): Standardizes toxicity value derivation using animal-to-human extrapolation (e.g., NOAEL → BMDL) .

  • Uncertainty Factors: Includes UFA (animal-human), UFS (subchronic-chronic), and UFL (LOAEL-NOAEL) to calibrate protective thresholds .

Ambiguities and Limitations

  • Terminology Conflicts: "CS" refers to multiple entities (e.g., conditioned stimuli, chondroitin sulfate, cigarette smoke), requiring context-specific interpretation.

  • Data Gaps: Few studies directly address "CS Human" as a standalone concept, necessitating interdisciplinary synthesis.

Product Specs

Introduction
Citrate synthase, also known as CS, is an enzyme involved in the Krebs tricarboxylic acid cycle. It catalyzes the reaction between oxaloacetate and acetyl coenzyme A to produce citrate. CS is found in most cells that utilize oxygen for energy production. It is encoded in the nucleus and transported to the mitochondria, where it becomes active. Deficiencies or dysfunctions in CS have been linked to medical conditions like critical illness polyneuropathy and mitochondrial cardiomyopathy.
Description
This product consists of the human CS enzyme, recombinantly produced in E. coli bacteria. It is a single, non-glycosylated polypeptide chain with 462 amino acids (specifically, amino acids 28 to 466) and a molecular weight of 51.4 kDa. For purification purposes, a 23 amino acid His-tag is attached to the N-terminus of the CS protein. The purification process utilizes proprietary chromatographic methods.
Physical Appearance
The product is a clear, colorless solution that has been sterilized through filtration.
Formulation
The CS protein is supplied in a solution with a concentration of 1 mg/ml. The solution is buffered with 20mM Tris-HCl at a pH of 8.0 and contains 0.15M NaCl, 10% glycerol, and 1mM DTT.
Stability
For optimal preservation of activity, store the product at 4°C if it will be used within 2-4 weeks. For longer storage periods, freezing at -20°C is recommended. Adding a carrier protein (0.1% HSA or BSA) can further enhance stability during long-term storage. To maintain product integrity, avoid repeated freeze-thaw cycles.
Purity
The purity of the CS protein is greater than 90%, as assessed by SDS-PAGE analysis.
Synonyms
Citrate Synthase, EC 2.3.3.1, Citrate (Si)-Synthase, EC 2.3.3, Citrate synthase, mitochondrial.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSASSTNLK DILADLIPKE QARIKTFRQQ HGKTVVGQIT VDMMYGGMRG MKGLVYETSV LDPDEGIRFR GFSIPECQKL LPKAKGGEEP LPEGLFWLLV TGHIPTEEQV SWLSKEWAKR AALPSHVVTM LDNFPTNLHP MSQLSAAVTA LNSESNFARA YAQGISRTKY WELIYEDSMD LIAKLPCVAA KIYRNLYREG SGIGAIDSNL DWSHNFTNML GYTDHQFTEL TRLYLTIHSD HEGGNVSAHT SHLVGSALSD PYLSFAAAMN GLAGPLHGLA NQEVLVWLTQ LQKEVGKDVS DEKLRDYIWN TLNSGRVVPG YGHAVLRKTD PRYTCQREFA LKHLPNDPMF KLVAQLYKIV PNVLLEQGKA KNPWPNVDAH SGVLLQYYGM TEMNYYTVLF GVSRALGVLA QLIWSRALGF PLERPKSMST EGLMKFVDSK SG.

Q&A

What is the fundamental relationship between computer science and humanities research?

Computer science and humanities are interlinked in more ways than one might imagine. The foundations of computer science lie in logic—without it, software developers could not fathom the most basic functions. Similarly, the origins of philosophical thought also lie in logic—what is an argument or a theory without elementary reasoning? With the exponential growth of artificial intelligence and technology, there is an increasing need for developers to have a deep understanding of ethics regarding technology, the inner workings of machines, and how they connect to human consciousness .

What academic paths are available for researchers interested in CS-Human interdisciplinary work?

Several paths exist within this interdisciplinary field, with the appeal lying in flexibility and innovation. Some researchers focus on the social aspects of information technology—specifically, policy impacts on human-technology interaction. Programs like CS + Philosophy allow researchers to gain deep understanding of niche fields and address policy issues in technology. Other routes include privacy and security research, modern questions about logic and ethics, or specialized work in cognition and artificial intelligence. Graduate programs in human-computer interaction, information science, and AI offer additional specialization opportunities for those pursuing advanced interdisciplinary research .

How do experimental designs differ between traditional CS research and human-centered CS research?

Human-centered CS research requires specific considerations that may not be prominent in traditional CS approaches. While traditional CS research might focus primarily on system performance metrics, human-centered research must account for human subject variability, ethical considerations, and mixed-methods approaches. Experimental designs typically include both quantitative and qualitative components, with careful attention to human-technology interaction patterns. Research approaches often involve case studies from human-computer interaction, natural language processing, and computer systems, demonstrating various methodologies including exemplary depth investigations, standard practices, innovative designs, and identification of unforeseen flaws .

What are the essential components of a well-designed CS-Human experiment?

Well-designed CS-Human experiments typically include several key components that ensure scientific rigor:

  • Clearly defined research questions and hypotheses

  • Appropriate participant selection and sampling methods

  • Control mechanisms for confounding variables

  • Proper experimental controls (e.g., Posttest Only Control Group Design or Pretest-Posttest Control Group Design)

  • Robust measurement tools and protocols

  • Appropriate statistical analysis methods

The evaluation section is particularly critical—papers often succeed or fail based on their evaluation methodology. Researchers must carefully select appropriate metrics, ensure statistical validity, and address potential confounding factors throughout the experimental process .

How should researchers approach the design of longitudinal studies in human-computer interaction?

Longitudinal research in human-computer interaction requires careful planning to capture changes over time. Researchers must consider:

  • Cross-sectional vs. longitudinal approaches: Cross-sectional research examines different age or user groups simultaneously, while longitudinal research follows the same subjects over time.

  • Sequential designs: These combine advantages of both cross-sectional and longitudinal methods.

  • Participant retention strategies: Methods to minimize attrition over the study period.

  • Consistent measurement: Ensuring instruments remain valid across different time points.

  • Analysis of cohort effects: Distinguishing between age effects, period effects, and cohort effects.

Researchers must understand the relative advantages and limitations of each approach. For example, cross-sectional studies are faster but may confuse age effects with cohort effects, while longitudinal studies provide more direct evidence of change but are more expensive and time-consuming .

What statistical approaches are most appropriate for analyzing data from CS-Human experiments?

The choice of statistical approach depends on the research design and data characteristics:

  • For experimental designs: ANOVA, t-tests, and ANCOVA are commonly used to compare groups.

  • For correlational designs: Pearson correlation, regression analysis, and structural equation modeling.

  • For categorical data: Chi-square tests and logistic regression.

  • For repeated measures designs: Repeated measures ANOVA or mixed-effects models.

When reporting results, researchers should include not only p-values but also effect sizes (e.g., Cohen's d, partial eta-squared, or correlation coefficients) to indicate practical significance. Effect size calculations vary by test type: for chi-square, effect size indicates strength of association; for F-statistics, measures like partial eta-squared quantify variance explained; for correlational analyses, the Pearson product-moment correlation coefficient serves as both test statistic and effect size measure .

How can researchers effectively address contradictions in dialogue modeling and human-computer interaction data?

Addressing contradictions in dialogue modeling is a complex challenge requiring specialized approaches. Researchers can implement structured methods utilizing Natural Language Inference (NLI) models to detect contradictions. In the unstructured approach, a Transformer NLI model directly processes the concatenation of all dialogue utterances. In the structured approach, utterances are paired separately before processing, explicitly accounting for dialogue structure.

Techniques to handle contradictions include:

  • DialoguE COntradiction DEtection (DECODE) frameworks that provide better supervision for contradiction detection.

  • Using both human-human and human-bot contradictory dialogues for training.

  • Implementing data augmentation techniques such as:

    • Add Random Turns (ART): Insert random utterances between contradicting utterances.

    • Remove Contradicting Turns (RCT): Remove turns marked as supporting evidence for contradictions.

These approaches help develop more consistent dialogue systems and better evaluate machine response quality in human-bot interactive conversations .

What methods exist for deepening extinction in human psychological experiments, and how do they relate to computational modeling?

In extinction studies related to fear conditioning (with applications in computational modeling of human behavior), deepened extinction is an emerging procedure based on maximizing surprise to promote loss of associative value of conditioned stimuli. The technique involves:

  • Initial acquisition phase: Multiple conditioned stimuli (CS) (e.g., a tone and a light) are separately paired with the same unconditioned stimulus (US) (e.g., an electric shock).

  • Isolated extinction phase: Individual stimuli undergo extinction.

  • Compound CS extinction phase: Combining previously extinguished and non-extinguished stimuli.

Research has shown two effective approaches:

  • Hendry method: Combining two previously extinguished CSs

  • Reberg method: Combining a previously extinguished CS with a non-extinguished one

Studies indicate the Reberg method provides more robust results for deepening extinction. In human experiments using intermodal cues (visual and auditory) with electrical shock, compound extinction significantly reduced spontaneous recovery 24 hours after treatment compared to traditional extinction methods .

How can researchers balance increasing CS enrollment demands with maintaining research quality in academic settings?

This challenge affects many CS departments nationwide. Based on experiences from academic institutions, several approaches can be implemented:

  • Resource allocation: Ensuring sufficient faculty members are available to teach courses while maintaining research activities.

  • Course prioritization: Guaranteeing required courses for majors have sufficient enrollment slots while potentially limiting electives.

  • Class size management: Maintaining smaller sizes for upper-division electives to preserve close working relationships with professors.

  • Long-term planning: Developing sustainable approaches to address increased enrollment without compromising education or research quality.

These challenges require institutional commitment and resources to ensure both educational and research needs are met. Departments must balance the immediate need to serve students with maintaining the research environment that makes the institution valuable in the first place .

How can researchers effectively design experiments that bridge computer science and humanities methodologies?

Bridging computer science and humanities methodologies requires intentional design choices:

  • Mixed-methods approaches: Combining quantitative methods typical in CS with qualitative methods common in humanities.

  • Interdisciplinary team composition: Including researchers with expertise in both domains.

  • Holistic measurement: Developing metrics that capture both technical performance and humanistic values.

  • Ethical frameworks: Incorporating ethical considerations throughout the research process.

  • Iterative design: Using feedback loops between technical development and humanistic inquiry.

Effective research in this space often requires experimental case studies from human-computer interaction, natural language processing, and computer systems. These case studies should include examples of exemplary depth, standard practices, innovative designs, and consideration of unforeseen ethical implications .

What research opportunities exist at the intersection of artificial intelligence and philosophical inquiry?

The intersection of AI and philosophy presents numerous research opportunities:

  • Ethics of AI: Examining ethical frameworks for AI development and deployment.

  • Consciousness and cognition: Exploring questions about machine consciousness and the nature of intelligence.

  • Epistemology: Investigating how AI systems "know" and the limits of machine knowledge.

  • Logic and reasoning: Developing formal systems that capture human-like reasoning patterns.

  • Social impact: Studying how AI technologies reshape human experience and society.

These areas require researchers to have deep understanding of both technical aspects of AI and philosophical frameworks. The exponential growth of artificial intelligence has increased the need for developers to understand the ethical implications of technology, the inner workings of machines, and how they connect to human consciousness .

How do experimental design requirements differ when studying dialogue systems versus studying human-human communication?

Studying dialogue systems versus human-human communication presents unique experimental design considerations:

AspectDialogue Systems ResearchHuman-Human Communication Research
ControlHigher system controlLower experimenter control
VariabilityMore predictable responsesHigher variability in responses
MeasurementFocus on technical metricsBalance of technical and social metrics
EthicsSystem privacy concernsHuman participant protections
ContextLimited contextual understandingRich contextual interpretation
Data collectionEasier to collect large datasetsMore resource-intensive data collection
AnalysisCan use automated analysisOften requires human coding

When designing experiments for dialogue systems, researchers must consider:

  • Out-of-distribution testing to evaluate system robustness

  • Human-in-the-loop evaluation procedures

  • Methods to detect and address contradictions in dialogue

  • Comparison of human-human and human-bot interactions

  • Structured approaches for analyzing dialogue coherence and consistency

What are the most effective experimental designs for evaluating human-computer interaction?

Effective experimental designs for human-computer interaction research must balance internal validity with ecological validity. Key designs include:

  • Posttest-Only Control Group Design: Participants are randomly assigned to experimental or control conditions and measured after intervention only.

  • Pretest-Posttest Control Group Design: Measurements are taken before and after intervention, allowing for analysis of change scores.

  • Quasi-Experimental Designs: Used when random assignment isn't possible, including:

    • One-Group Pretest-Posttest Design

    • Nonequivalent Control Group Design

Well-designed experiments incorporate appropriate controls, randomization where possible, and careful consideration of potential confounding variables. Researchers must diagram their designs to clearly communicate the experimental structure, including the relationship between independent and dependent variables .

How can researchers effectively handle contradictions in collected data when studying human interactions with technology?

Addressing contradictions in human-technology interaction data requires systematic approaches:

  • Data triangulation: Using multiple data sources to verify findings

  • Mixed-methods validation: Comparing quantitative measurements with qualitative insights

  • Statistical techniques: Identifying outliers and determining whether they represent measurement error or important findings

  • Structured analysis frameworks: Employing specific methodologies for contradiction detection

  • Version control: Maintaining clear documentation of all data transformations

When studying dialogue systems specifically, researchers can implement specialized techniques like those in the DECODE framework to systematically identify and address contradictions in conversational data, improving both research validity and system performance .

Product Science Overview

Introduction

Citrate synthase is a crucial enzyme in the citric acid cycle (Krebs cycle), which is fundamental for cellular respiration in nearly all living cells. This enzyme catalyzes the synthesis of citrate from oxaloacetate and acetyl coenzyme A, marking the first step of the citric acid cycle .

Structure and Function

Citrate synthase is located within the mitochondrial matrix of eukaryotic cells. Despite its mitochondrial function, it is encoded by nuclear DNA and synthesized using cytoplasmic ribosomes. Once synthesized, it is transported into the mitochondrial matrix where it becomes functional . The enzyme’s activity is often used as a quantitative marker for the presence of intact mitochondria .

The enzyme catalyzes the following reaction:

acetyl-CoA+oxaloacetate+H2Ocitrate+CoA-SH\text{acetyl-CoA} + \text{oxaloacetate} + \text{H}_2\text{O} \rightarrow \text{citrate} + \text{CoA-SH}

This reaction is essential for the continuation of the citric acid cycle, which is pivotal for energy production in cells .

Recombinant Human Citrate Synthase

Recombinant human citrate synthase is produced using various expression systems, such as E. coli or baculovirus-insect cells. The recombinant protein typically includes a polyhistidine tag to facilitate purification. For instance, a recombinant human citrate synthase produced in E. coli consists of 462 amino acids and has a molecular mass of approximately 51.4 kDa .

Applications

Recombinant citrate synthase is widely used in biochemical research to study mitochondrial function and energy metabolism. It is also employed in various assays to measure the activity of the citric acid cycle and to investigate mitochondrial integrity and function .

Stability and Storage

Recombinant citrate synthase is generally provided as a lyophilized powder, which is stable for up to twelve months when stored at -20°C to -80°C. It is recommended to aliquot the protein to avoid repeated freeze-thaw cycles, which can degrade the protein .

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