CSB Antibody

Definition and Function of CSB Protein

The CSB protein (ERCC6) is a SWI/SNF-like ATPase involved in maintaining chromatin structure and resolving transcription-associated DNA damage . Mutations in the CSB gene cause Cockayne syndrome (CS), a rare genetic disorder characterized by premature aging, neurodegeneration, and cancer susceptibility .

Types of CSB Antibodies

CSB antibodies are categorized into polyclonal and monoclonal variants, each with distinct advantages:

Key Features:

• Polyclonal antibodies (e.g., GTX104589) offer broad epitope recognition but may have higher background noise .

• Recombinant monoclonal antibodies (VeRSaMAb) are engineered for specificity and reproducibility, eliminating lot-to-lot variability .

Applications in Research

CSB antibodies are critical tools in studying DNA repair, chromatin dynamics, and neurodegenerative diseases.

Western Blotting (WB)

• Detects CSB protein in nuclear extracts (e.g., 1:1000 dilution for GTX104589) .

• Validates CSB knockdown in cellular models (e.g., siERCC6-treated cells) .

Immunofluorescence (ICC/IF)

• Localizes CSB to nuclear foci during DNA damage response .

• Visualizes R-loop accumulation in TOP2-inhibited cells .

Immunoprecipitation (IP)

• Identifies CSB interactions with BRCA1, MRN complex, and CtIP .

• Confirms strand-annealing activity in CSB immunodepletion assays .

DNA Repair and Chromatin Remodeling

• CSB facilitates repair of oxidative DNA lesions (8-oxoguanine) via BER pathways .

• Promotes resolution of DNA interstrand crosslinks (ICLs) by coordinating with SNM1A .

Mitochondrial Function

• CSB knockdown leads to mitochondrial dysfunction, including reduced POLG1 levels and increased protease activity (HTRA2/3) .

Transcription-Associated Damage

• Stimulates TOP2A/B activity to prevent R-loop/DSB accumulation .

• Interacts with BRCA1 in late S/G2 phase to regulate MRN-CtIP-mediated repair .

Data Table: Antibody Performance