csl4 Antibody

Overview of CSL4 Antibody

The CSL4 antibody (e.g., ab181108 from Abcam) is a rabbit monoclonal antibody targeting the human CSL4 protein. It is validated for multiple applications, including Western blotting (WB), immunohistochemistry (IHC), and immunofluorescence (IF). The antibody recognizes a predicted molecular weight of 21 kDa, with observed bands at 21–22 kDa in various cell lysates .

2.1. Western Blot Analysis

The antibody demonstrates consistent performance across diverse cell lines under reducing conditions:

Key observations:

• Specificity confirmed via immunoprecipitation in Raji lysate .

• Negative controls (e.g., C6, Raw264.7) show no cross-reactivity .

2.2. Immunohistochemistry (IHC)

• Human cervix tissue: Strong cytoplasmic staining at 1/100 dilution after EDTA-based antigen retrieval .

• Human colon tissue: Distinct membranous/cytoplasmic localization under similar conditions .

2.3. Immunofluorescence (IF)

• 293 cells: Clear nuclear and cytoplasmic labeling at 1/250 dilution, validated with DAPI counterstaining .

Technical Validation and Quality Control

• Host species: Rabbit (IgG isotype).

• Epitope: Linear peptide within human CSL4.

• Cross-reactivity: No significant reactivity with mouse or rat CSL4 homologs reported .

• Storage: Stable at 4°C for short-term; long-term storage recommended at -20°C .

Comparative Notes on Antibody Engineering

While not directly related to CSL4, broader antibody design principles (e.g., Fc engineering, hinge region optimization) highlight the importance of structural features in minimizing off-target effects . For example, Fc silencing (e.g., removal of ADCC/ADCP activity) in antibodies like cadonilimab underscores industry trends relevant to therapeutic antibody development.

Limitations and Future Directions