Customize CSLD2 Antibody

Description

Biological Role of CSLD2

CSLD2 is a member of the Cellulose Synthase-Like D (CSLD) family in Arabidopsis, functioning as a mannan synthase critical for cell wall biosynthesis during plant development. It operates cooperatively with CSLD3 and CSLD5, forming heteromeric complexes essential for synthesizing cell wall polysaccharides required for cytokinesis and root hair elongation .

Mutant Phenotype	Developmental Defects	Key References
csld2 single mutant	Root hair rupture during late development; partial recovery of tip growth post-rupture
csld2/csld3 double mutant	Synergid cell dysfunction, reduced fertility
csld2/csld5 double mutant	Severe dwarfism, reduced viability

Molecular Interactions and Complex Formation

CSLD2 interacts directly with CSLD3 and CSLD5, as shown by bimolecular fluorescence complementation assays. These interactions suggest a functional complex critical for mannan synthesis. Additionally, CSLD2 is regulated by the anaphase-promoting complex (APC) activator CCS52A2, which mediates its ubiquitination and degradation during cell cycle progression .

Key Findings:

CSLD2 activity is interdependent with CSLD3 and CSLD5 for cell plate formation during mitosis .
Yeast two-hybrid assays confirm direct interaction between CSLD2 and CCS52A2, linking its turnover to cell cycle regulation .
CSLD2 lacks G2/M-phase-specific transcriptional regulation, unlike CSLD5, indicating functional divergence .

Applications of CSLD2 Antibody in Research

While no studies explicitly describe the development or validation of a CSLD2-specific antibody, inferred applications include:

Localization Studies: Tracking CSLD2 during cell plate formation using fluorescent tagging (e.g., Cerulean-CSLD2 fusions) .
Protein Turnover Analysis: Immunoprecipitation with anti-ubiquitin antibodies to study APC-mediated degradation .
Mutant Phenotyping: Western blotting to quantify CSLD2 levels in csld mutants .

Research Gaps and Future Directions

Antibody Validation: No peer-reviewed publications yet detail CSLD2 antibody specificity or epitope mapping.
Functional Redundancy: Overlapping roles of CSLD2 with CSLD3/CSLD5 complicate isolation of its unique contributions .
Evolutionary Conservation: CRISPR/Cas9 editing of SoCSLD2 in spinach highlights conserved roles in root hair development, suggesting cross-species antibody utility .

Product Specs

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Lead Time

Made-to-order (14-16 weeks)

Synonyms

CSLD2 antibody; At5g16910 antibody; F2K13.60Cellulose synthase-like protein D2 antibody; AtCslD2 antibody; EC 2.4.1.- antibody

Target Names

CSLD2

Uniprot No.

Q9LFL0

Target Background

Function

CSLD2 is hypothesized to be a Golgi-localized beta-glycan synthase responsible for polymerizing the backbones of noncellulosic polysaccharides (hemicelluloses) within the plant cell wall.

Gene References Into Functions

Research indicates that the N-terminus of the Arabidopsis cellulose synthase-like D2 protein likely faces the cytosol. PMID: 18642024
CSLD2 plays a crucial role in a later stage of hair development compared to CSLD3. Mutations in CSLD2 lead to root hairs exhibiting a range of abnormalities, with many experiencing rupture during later developmental stages. PMID: 18768911

Database Links

KEGG: ath:AT5G16910

STRING: 3702.AT5G16910.1

UniGene: At.20318

Protein Families

Glycosyltransferase 2 family, Plant cellulose synthase-like D subfamily

Subcellular Location

Golgi apparatus membrane; Multi-pass membrane protein.

Q&A

Based on the provided search results and absence of direct references to "CSLD2 Antibody" in the literature corpus, this term appears to be either a typographical error or a highly specialized/non-canonical designation not represented in current antibody research databases. Below is an analytical framework for addressing such terminology gaps in antibody research:

Advanced Methodological Challenges

How to design experiments for uncharacterized antibody targets?
Stepwise approach:

Epitope characterization:
- Use phage display libraries to map potential binding regions
- Combine hydrogen-deuterium exchange mass spectrometry (HDX-MS) with cryo-EM for structural insights

Functional validation:

Assay Type	Purpose	Validation Controls
Surface plasmon resonance (SPR)	Binding kinetics	Known antigen-antibody pairs
Flow cytometry	Cellular localization	Isotype-matched antibodies
Immunoprecipitation	Complex identification	Knockout cell lines

How to address conflicting data in antibody specificity studies?
- Implement orthogonal validation methods:
  - Compare ELISA vs. antigen-binding bead assays for conformational vs linear epitopes
  - Use CRISPR-edited cell lines as negative controls
  - Apply deep learning models for specificity prediction (AUC >0.85 required)

Key Technical Considerations

What experimental factors affect antibody reproducibility?
- Critical variables:
  - Somatic hypermutation patterns in recombinant antibodies
  - Post-translational modifications (e.g., glycosylation)
  - Storage conditions impacting conformational stability

Emerging Methodologies

How to leverage public antibody response data for novel target research?
- Analyze recurrent features in 8,000+ antibody sequences:
  - IGHD gene usage patterns (e.g., IGHD1–26 in S2 antibodies)
  - CDR H3 length distributions (14aa public response signature)
  - Somatic hypermutation hotspots (e.g., VH3-53 in SARS-CoV-2 RBD antibodies)
- Apply deep learning frameworks trained on viral antigen responses

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CSLD2 Antibody