CSNK1A1 Antibody
Product Specs
Target Background
- Research suggests that CK1α similarly destabilizes FOXO4 in RAS-mutant cells through phosphorylation at serines 265/268. PMID: 28945225
- Data indicate that CK1α RNA levels could serve as a prognostic marker for colorectal cancer (CRC). PMID: 29409464
- CK1α, a cellular kinase, is crucial for IAV HA-induced degradation of both IFNGR1 and IFNAR1. PMID: 29343571
- Casein kinase 1 phosphorylates the SQSTM1 S349 residue when harmful proteins accumulate under HSF1 stress. PMID: 27846364
- Maintenance of progenitors necessitates collaboration between PRMT1 and CSNK1a1 to sustain proliferation gene expression and suppress premature differentiation driven by GRHL3. PMID: 28943242
- These data suggest that CK1α plays a dominant and non-redundant role in melanoma cells. PMID: 27488834
- Disrupting CK1α function in myeloma cells resulted in decreased Mdm2, increased p53 and p21, and reduced expression of beta-catenin and AKT. PMID: 28099937
- CK1δ enhances EPO secretion from liver cancer cells under hypoxia by modifying HIF-2α and promoting its nuclear accumulation. PMID: 27686097
- CKIα-mediated NS5A S235 phosphorylation is essential for HCV replication. CaMKII gamma and delta may have negative roles in the HCV life cycle. PMID: 27875595
- Interactome analyses revealed that the Jade-1S mutant, unable to be phosphorylated by CK1α, exhibits increased binding affinity to proteins involved in chromatin remodeling, histone deacetylation, transcriptional repression, and ribosome biogenesis. PMID: 26919559
- This study investigates how the structural dynamics and conformational changes of two CK1 isoforms are synchronized in the carcinogenic pathway. PMID: 26788877
- NIFK is required for lung cancer progression through RUNX1-dependent CK1α repression, which activates TCF4/beta-catenin signaling in metastasis and Ki-67-dependent regulation in cell proliferation. PMID: 26984280
- The study describes the identification of a novel, tumor-specific missense mutation in the active site of casein kinase 1 alpha (CSNK1A1) using activity-based proteomics. PMID: 27031502
- This research characterizes the frequency and potential clinical impact of CSNK1A1 mutations in myelodysplastic syndrome (MDS) and acute myeloid leukemia following MDS in a large patient cohort. PMID: 25792355
- The dynamical and conformational properties of three CK1 isoforms are explored through molecular dynamics (MD) simulations. PMID: 25665722
- Phosphorylation of LRRK2 by casein kinase 1α regulates trans-Golgi clustering through differential interaction with ARHGEF7. PMID: 25500533
- Casein kinase 1α is a key negative regulator of oncogenic RAS-induced autophagy. Depletion or pharmacologic inhibition of CK1α enhanced autophagic flux in oncogenic RAS-driven human fibroblasts and multiple cancer cell lines. PMID: 25798617
- CSNK1alpha1 plays a role in mediating malignant plasma cell survival. PMID: 24962017
- Casein kinase 1α specifically phosphorylates Jade-1 at an unprimed SLS phosphorylation site. PMID: 25100726
- Degradation of Tiam1 by casein kinase 1 and the SCFbetaTrCP ubiquitin ligase controls the duration of mTOR-S6K signaling. PMID: 25124033
- Data indicate that protein S phosphorylation by casein kinase 1 (CK1) and casein kinase 2 (CK2) increased activated proten C cofactor activity. PMID: 23580615
- The substrate interacting residues in ck1α have been identified using the structural model of kinase - substrate peptide. The PKR interacting NS5A 1b residues have also been predicted. PMID: 23148689
- DNA damage activates p53, in part, by disrupting CK1α-MDMX interaction and reducing MDMX-p53 binding affinity. PMID: 23028042
- Casein kinase 1α (CK1α) was identified as a direct target of miR-155 control, which enhanced beta-catenin signaling and cyclin D1 expression, promoting tumor cell growth. PMID: 22350414
- Just four genes, G3BP2, SCARB2, CSNK1A1, and SPRR2B, can classify patients as having lymph node metastasis negative or positive, with 80.0% accuracy. PMID: 21985131
- mTOR cooperates with CK1α and betaTrCP to create an auto-amplification loop promoting its own full activation. PMID: 22017877
- Researchers characterized the role of CKIα in the clock mechanism and found that CKIα-mediated phosphorylation stimulated degradation of a clock protein PER1, similar to the function of CKIδ. PMID: 21179498
- Findings indicate that the nuclear form of CK1α in humans, CK1αLS, plays a critical role in vascular cell proliferation, cellular activation, and hydrogen peroxide-mediated mitogenic signal transduction. PMID: 20696773
- Casein kinase 1 functions as both the penultimate and ultimate kinase in regulating Cdc25A destruction. PMID: 20348946
- Biochemical and cellular characteristics of the four splice variants of protein kinase CK1α from zebrafish (Danio rerio) are presented. PMID: 12210746
- CK1α can promote cell survival by interfering with RXR agonist-induced apoptosis. PMID: 15131121
- CK1α-mediated phosphorylation modulates the mRNA binding ability of hnRNP-C. PMID: 15687492
- NFAT1, a transcription factor associated with breast cancer metastasis, is activated by Wnt-5a through a Ca2+ signaling pathway in human breast epithelial cells, which was simultaneously counteracted by a Wnt-5a-induced Yes/Cdc42 signaling pathway. PMID: 16880514
- The alpha isoform of casein kinase I was identified as the cellular kinase critical for the formation of hepatitis C virus (HCV) NS5A-p58; this research demonstrates the effects of this kinase on NS5A hyperphosphorylation and HCV replication. PMID: 16943283
- CK1αLS and hnRNP-C represent conserved components of a vertebrate-specific H(2)O(2)-responsive nuclear signaling pathway. PMID: 17626781
- Studies suggest that CK1α is another kinase that regulates RIP1 function in NF-kappaB activation. PMID: 18067272
- Following DNA damage, PML facilitates Thr18 phosphorylation by recruiting p53 and CK1 into PML nuclear bodies, thereby protecting p53 from inhibition by Mdm2, leading to p53 activation. PMID: 18246126
- Ser-155 and Ser-158 play crucial roles in the nucleocytoplasmic trafficking of OREBP/TonEBP, and CK1 plays a significant role in regulating this process. PMID: 18411282
- This research demonstrates that inhibiting CK1 activates RhoB and promotes RhoB-dependent actin fiber formation and EGF-R level. PMID: 18590726
- CK1 plays a role as the Ser20 site kinase for p53 in DNA virus-infected cells. PMID: 18669630
- Phosphorylation of parkin by casein kinase I and cdk5 decreases parkin solubility, leading to its aggregation and inactivation. PMID: 19050041
- The 'activated B-cell-like' subtype of diffuse large B-cell lymphoma cells required CK1α for constitutive NF-kappaB activity, indicating that CK1α functions as a conditionally essential malignancy gene. PMID: 19118383
- Casein Kinase 1 phosphorylates serine 56 of yellow fever virus methyltransferase. PMID: 19185594
- Data show that Wnt-5a stimulation of breast epithelial cells leads to increased cell-cell adhesion, and that Wnt-5a/casein kinase Iα (CKIα)-specific phosphorylation of beta-catenin leads to increased complex formation of beta-catenin/E-cadherin. PMID: 19244247
- CK1α plays a central role in mediating MDM2 control of p53 and E2F-1 protein stability. PMID: 19759023
- Results suggest a role for mammalian and parasite casein kinase 1α in regulating IFNAR1 stability and type I IFN signaling. PMID: 19805514
Q&A
What experimental strategies validate CSNK1A1 antibody specificity in western blotting?
Validating antibody specificity requires a multi-step approach:
-
Knockout/Knockdown Controls: Compare wild-type (WT) and CSNK1A1 knockout (KO) or siRNA-treated cell lysates. A study screening ten commercial antibodies demonstrated that only 40% showed complete signal loss in KO lysates, underscoring the necessity of genetic controls .
-
Band Pattern Analysis: CSNK1A1 migrates at ~37 kDa. Researchers should verify absence of non-specific bands at 55 kDa (common cross-reactivity with CSNK1D/E isoforms) .
-
Orthogonal Validation: Combine western blot with immunoprecipitation-mass spectrometry to confirm target identity, as implemented in standardized antibody characterization pipelines .
Key Validation Data from Recent Studies
| Antibody Clone | KO Validation | Off-Target Bands | Recommended Dilution |
|---|---|---|---|
| CAB9308 | Complete | None observed | 1:500 - 1:2000 |
| 55192-1-AP | Partial | 55 kDa band | 1:1000 |
| ab196684 | Not tested | Uncharacterized | 1:50 (IHC) |
How does CSNK1A1 regulate Wnt/β-catenin signaling, and which antibody applications best capture this interaction?
CSNK1A1 phosphorylates β-catenin at Ser45, initiating its degradation via the β-TrCP ubiquitin ligase complex . To study this:
-
Co-Immunoprecipitation (Co-IP): Use antibodies validated for IP (e.g., CAB9308 ) to pull down CSNK1A1-β-catenin complexes from Wnt3a-stimulated cells.
-
Subcellular Localization: High-performance IF antibodies (e.g., those showing >5-fold signal reduction in KD cells ) reveal cytoplasmic-to-nuclear β-catenin shifts upon CSNK1A1 inhibition.
-
Phospho-Specific Assays: Pair CSNK1A1 antibodies with phospho-β-catenin (Ser45) reagents to quantify kinase activity dynamics.
What cell lines are optimal for CSNK1A1 functional studies?
Selection criteria include:
-
Endogenous Expression: HCT 116 colorectal cancer cells show highest CSNK1A1 levels (DepMap TPM >8.5) .
-
Knockout Viability: Use inducible KO systems, as constitutive CSNK1A1 knockout is lethal in many lines .
-
Pathway Activity: SW480 (APC mutant) cells provide high β-catenin background to assess CSNK1A1-mediated degradation .
How should researchers resolve contradictory CSNK1A1 localization data between immunofluorescence and biochemical fractionation?
Contradictions often arise from:
-
Fixation Artifacts: Methanol fixation may leak soluble CSNK1A1; compare with paraformaldehyde results .
-
Antibody Epitope Accessibility: Screen multiple clones (e.g., N-terminal vs. C-terminal epitopes) across fixation/permeabilization conditions .
-
Fractionation Validation: Verify cytoplasmic/nuclear separation using Lamin B1 and GAPDH controls. A 2024 study found only 60% of antibodies reproduced localization across methods .
What multiplex assay designs improve CSNK1A1 pathway analysis?
Innovative approaches include:
-
Mosaic Culture IF: Label WT and CSNK1A1 KD HCT 116 cells with CellTracker Green/Deep Red, then co-culture and stain in situ to control for batch effects .
-
Phosphoproteomics Integration: Combine IP-validated antibodies (Table 1) with TiO2 phosphopeptide enrichment to map CSNK1A1 substrate networks.
-
Live-Cell Biosensors: Use CSNK1A1 antibody-validated KO lines to calibrate FRET reporters for real-time β-catenin degradation kinetics.
Multiplex Workflow Validation Metrics
Which experimental parameters most significantly impact CSNK1A1 antibody performance in immunohistochemistry?
Critical factors identified in multi-laboratory studies:
-
Epitope Stability: Prolonged formalin fixation (>48 hrs) masks conformational epitopes; optimize retrieval pH (citrate pH6 vs. Tris-EDTA pH9) .
-
Phosphorylation State: Commercial antibodies show up to 40% reduced affinity for autophosphorylated CSNK1A1 . Include lambda phosphatase-treated controls.
-
Cell Cycle Synchronization: CSNK1A1 levels vary 2.3-fold between G1/S phases; standardize harvest times .
How can researchers address CSNK1A1 antibody lot-to-lot variability in longitudinal studies?
Mitigation strategies:
-
Batch Testing: Pre-screen new lots against reference samples (WT/KO lysates, peptide arrays).
-
Epitope Mapping: Select antibodies targeting linear epitopes (e.g., CAB9308 ) rather than conformational.
-
Public Data Utilization: Cross-reference with characterization databases (YCharOS, Antibody Registry) for performance benchmarks .
