At1g03700 Antibody

Applications and Usage

The antibody is validated for ELISA and Western Blot to detect the At1g03700 protein in Arabidopsis thaliana samples . It is critical for studies involving:

• Protein localization: Tracking spatial expression patterns in plant tissues.

• Gene function analysis: Correlating protein levels with phenotypic changes in mutant or transgenic plants.

• Biochemical assays: Investigating interactions with other cellular components.

Research Context

While no direct studies using this antibody are cited in the provided sources, the At1g03700 gene is part of Arabidopsis thaliana’s genome, a model system for plant biology. Antibodies like this are typically employed to explore roles in developmental processes, stress responses, or metabolic pathways.

Limitations and Considerations

• Specificity: Polyclonal antibodies may exhibit cross-reactivity; validation with knockout controls is recommended.

• Commercial Availability: Produced as a made-to-order reagent, requiring advance planning for experimental workflows .

Comparative Analysis with Other Antibodies

Unlike AT1 receptor antibodies (e.g., AT1R Abs in human studies ), the At1g03700 Antibody is plant-specific and non-pathogenic in scope. Notably, commercial antibodies targeting GPCRs like AT1R have faced validation challenges , underscoring the importance of rigorous specificity testing for all research antibodies.

Future Directions

Further studies using this antibody could elucidate the At1g03700 protein’s role in Arabidopsis thaliana, potentially contributing to broader agricultural or biochemical insights. Researchers are advised to pair this tool with genomic or proteomic datasets for comprehensive analyses.