At1g49405 Antibody

Analysis of the Query Term "At1g49405"

• Nomenclature Context:

  • The prefix "At" typically denotes Arabidopsis thaliana (a model plant organism).

  • "1g49405" follows standard Arabidopsis gene identifiers (chromosome 1, gene 49405).

  • No characterized protein or antibody linked to this locus exists in public repositories.

AT1R Antibody Research Findings

Critical Challenges in Antibody Validation

• Non-Specific Binding: Commercially available AT1R antibodies (e.g., sc-1173, ab9391) detect off-target bands in Western blots and show inconsistent immunostaining patterns unrelated to AT1R expression .

• Preabsorption Tests: While preabsorption with antigen peptides eliminates staining, this does not confirm specificity, as demonstrated in AT1R-knockout models .

Recommendations for Future Research

1. Clarify Target Identity: Verify the correct identifier (e.g., AT1R vs. hypothetical At1g49405).

2. Antibody Validation: Use knockout controls and functional assays to confirm specificity.

3. Explore Alternatives: Radioligand binding remains the gold standard for AT1R studies due to antibody reliability issues .