At4g03540 Antibody

Target Protein: At4g03540

The At4g03540 gene encodes a dirigent-like protein involved in disease resistance mechanisms in Arabidopsis thaliana. Dirigent proteins are known to mediate lignan biosynthesis and plant defense responses . The protein’s structure includes conserved domains typical of dirigent family members, which facilitate stereoselective coupling of monolignol radicals during lignin formation .

Research Applications

• ELISA: Used for quantitative detection of the At4g03540 protein in plant extracts .

• Western Blot: Validates protein expression levels in transgenic or mutant Arabidopsis lines .

• Functional Studies: Investigates the role of dirigent-like proteins in plant-microbe interactions and lignin biosynthesis .

Immunogen Design and Validation

The immunogen is a recombinant protein derived from the full-length At4g03540 sequence. Specificity is confirmed through:

• Cross-reactivity tests against related dirigent proteins.

• Immunoblotting using Arabidopsis wild-type and mutant samples .

Limitations and Considerations

• Species Specificity: Reactivity is confirmed only in Arabidopsis thaliana; cross-species applications require validation .

• Protein Stability: Target protein expression may vary under stress conditions, necessitating optimized extraction protocols .