CST9L Antibody
Description
Structure and Immunogen Details
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Antigen Target: CST9L is a 147-amino-acid secreted protein linked to cysteine protease inhibition, particularly in hematopoietic differentiation and inflammation .
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Immunogen: Synthesized peptide derived from the internal region of human CST9L (Q9H4G1) .
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Purification: Affinity chromatography using epitope-specific immunogens ensures >95% purity (by SDS-PAGE) .
Applications
The CST9L antibody is validated for:
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Immunohistochemistry (IHC): Stains CST9L in paraffin-embedded tissue sections (1:50–1:100 dilution) .
| Application | Dilution | Species Reactivity |
|---|---|---|
| ELISA | 1:10,000 | Human, Mouse, Rat |
| Immunohistochemistry | 1:50–1:100 | Human, Rat |
| Western Blot | 1:1,000–1:5,000 | Human, Mouse |
Antibody Specificity
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Knockdown Validation: siRNA-mediated silencing of CST9L in SH-SY5Y cells reduced antibody signal intensity in Western blot .
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Cross-Reactivity: No significant binding to non-target proteins in ELISA or IHC .
Performance in Engineered Systems
While the CST9L antibody is not engineered, studies on recombinant antibodies (e.g., Parkin/OCT4) demonstrate that Fc domain engineering can enhance sensitivity by 2-fold in Western blot and 1.5-fold in immunocytochemistry . Such modifications could theoretically improve CST9L antibody performance.
Product Specs
Target Background
- Phylogenetic analysis reveals that CTES genes are clustered into three distinct groups: testatin, Cres, and CstL1. (PMID: 20565543)
- This study characterizes the sequence, genomic structure, chromosomal location, and expression pattern of the human testatin gene. (PMID: 11756564)
