CTBP1 Antibody
Description
CTBP1 Antibody Characteristics
CTBP1 antibodies are designed to detect the 48 kDa protein encoded by the CTBP1 gene. These antibodies are available in polyclonal and monoclonal formats, with varying specificities and reactivities.
Applications of CTBP1 Antibodies
CTBP1 antibodies are utilized across multiple experimental platforms to study protein localization, interactions, and functional roles.
Western Blot (WB)
CTBP1 antibodies detect a band at ~48 kDa under reducing conditions. Key findings include:
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Detection in cell lines: HEK-293, HepG2, Jurkat, SH-SY5Y, and HeLa cells .
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Tissue reactivity: Mouse skeletal muscle, thymus, brain, and human prostate cancer tissues .
Immunohistochemistry (IHC)
Antigen retrieval with TE buffer (pH 9.0) or citrate buffer (pH 6.0) is recommended. Applications include:
Immunofluorescence (IF) and Flow Cytometry (FC)
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Presynaptic localization: CTBP1 colocalizes with Bassoon in presynaptic terminals .
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Intracellular detection: Used in HepG2 cells and Raji lymphoma cells .
Research Findings and Functional Insights
CTBP1 antibodies have elucidated critical roles of CTBP1 in:
Metabolic Regulation
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NADH/NAD⁺ sensing: CTBP1 binds NADH, influencing gene silencing and metabolic pathways .
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Lipid metabolism: Overexpression in granulosa cells reduces aromatase (CYP19A1) expression, linking to polycystic ovary syndrome (PCOS) and dyslipidemia .
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Adipose tissue browning: CTBP1 inhibition promotes white adipose tissue browning via β3-adrenergic signaling .
Synaptic Plasticity and Neurodevelopment
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Activity-dependent shuttling: CTBP1 redistributes between presynapses and nuclei in response to neuronal activity, modulating genes like ARC, BDNF, and EGR1 .
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Brain development: Expressed in embryonic telencephalon, oligodendrocytes, and Purkinje cells, suggesting roles in myelination and cerebellar function .
Transcriptional Repression
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Corepressor activity: CTBP1 recruits histone-modifying enzymes to repress genes involved in epithelial-mesenchymal transition and oncogenesis .
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Polycomb interactions: Associates with polycomb group proteins to regulate developmental gene expression .
CTBP1 Antibody Performance Across Platforms
The table below summarizes key performance metrics for commercially available CTBP1 antibodies:
Clinical and Diagnostic Relevance
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PCOS and Metabolic Syndrome: Elevated CTBP1 in granulosa cells correlates with reduced estradiol and lipid dysregulation .
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Neurodevelopmental Disorders: Mutations in CTBP1 are linked to Wolf-Hirschhorn syndrome and HADDTS .
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Cancer Research: CTBP1 interacts with BRCA1 and polycomb complexes, implicating it in oncogenesis and DNA repair .
Product Specs
Product Science Overview
C-Terminal Binding Protein 1, also known as CtBP1, is a transcriptional co-repressor that plays a significant role in various cellular processes, including gene regulation, cell survival, and development. The monoclonal mouse anti-human antibody targeting CtBP1 is a crucial tool in biomedical research, particularly in the study of cancer and other diseases where CtBP1 is implicated.
CtBP1 belongs to the hydroxyacid-dehydrogenase family and possesses a NAD(H)-binding Rossmann fold. This structural feature is essential for its function as it allows CtBP1 to oligomerize and interact with various protein partners. CtBP1 acts as a transcriptional co-repressor by binding to specific DNA sequences and recruiting other proteins to form a repressive complex. This complex inhibits the transcription of target genes, thereby regulating various cellular processes.
CtBP1 is overexpressed in multiple cancers and has pro-tumorigenic functions. It regulates gene networks associated with cancer hallmarks, such as increased cell survival, proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT). By repressing the expression of tumor suppressor genes and promoting the expression of oncogenes, CtBP1 contributes to the malignant behavior of cancer cells .
The monoclonal mouse anti-human antibody targeting CtBP1 is developed to specifically bind to the human CtBP1 protein. This antibody is produced by immunizing mice with human CtBP1 protein, followed by the isolation and cloning of specific B cells that produce the desired antibody. The monoclonal nature of the antibody ensures high specificity and affinity for the target protein, making it a valuable tool for research and therapeutic applications.
- Cancer Research: The monoclonal mouse anti-human CtBP1 antibody is widely used in cancer research to study the role of CtBP1 in tumorigenesis. It helps in identifying the expression levels of CtBP1 in various cancer types and understanding its contribution to cancer progression.
- Gene Regulation Studies: Researchers use this antibody to investigate the mechanisms by which CtBP1 regulates gene expression. By identifying the target genes and interacting partners of CtBP1, scientists can gain insights into the complex regulatory networks controlled by this protein.
- Drug Development: The antibody serves as a tool for screening potential inhibitors of CtBP1. By targeting the CtBP1 protein, researchers aim to develop novel therapeutic strategies for cancers and other diseases where CtBP1 plays a critical role.
