CSTF64 Antibody

Definition and Biological Role

CSTF64 Antibody detects the CSTF64 protein, which recognizes GU-rich elements downstream of polyadenylation sites (PAS) in pre-mRNAs. CSTF64 is part of the cleavage stimulation factor (CSTF) complex, essential for mRNA 3ʹ-end cleavage and polyadenylation . This process ensures proper mRNA maturation and stability, influencing gene expression regulation .

Applications in Research

CSTF64 Antibody is widely used for:

Regulatory Role in APA

• CSTF64 and CSTF64τ exhibit functional redundancy in PAS selection. Codepletion in HeLa cells caused a proximal-to-distal PAS shift in 85% of affected genes, indicating their compensatory roles .

• CSTF64 binds dormant intronic PASs suppressed by U1 snRNP, preventing premature transcription termination .

Role in Cellular Differentiation

• Macrophage Differentiation:

  • CSTF64 knockdown (KD) in U937 cells reduced CD68/CD38 (macrophage markers) and increased CD14 (monocyte marker), impairing differentiation .

  • Overexpression (OE) enhanced macrophage marker expression and morphological complexity .

Disease Relevance

• CSTF64 overexpression promotes lung cancer proliferation .

• Dysregulation linked to neurodegeneration and cardiac fibrosis .

Validation and Specificity

• Specificity Confirmation: Dual-color Western blotting distinguishes CSTF64 (64 kDa) and CSTF64τ (70 kDa) in human/mouse cells .

• Functional Validation: RNAi-mediated CSTF64 depletion confirmed antibody specificity via reciprocal CSTF64τ upregulation .