CSTF64 Antibody

Shipped with Ice Packs
In Stock

Description

Definition and Biological Role

CSTF64 Antibody detects the CSTF64 protein, which recognizes GU-rich elements downstream of polyadenylation sites (PAS) in pre-mRNAs. CSTF64 is part of the cleavage stimulation factor (CSTF) complex, essential for mRNA 3ʹ-end cleavage and polyadenylation . This process ensures proper mRNA maturation and stability, influencing gene expression regulation .

Applications in Research

CSTF64 Antibody is widely used for:

ApplicationDescriptionExamples from Literature
Western Blot (WB)Detects CSTF64 (~64 kDa) and CSTF64τ (~70 kDa) in human/mouse cell lines .Validation in HeLa, COLO 320, and mouse tissues .
Immunoprecipitation (IP)Identifies CSTF64-protein interactions (e.g., with symplekin) .Co-IP studies in HeLa cells .
Immunohistochemistry (IHC)Localizes CSTF64 in tissues (e.g., breast cancer) .Detection in human tumor samples .
Functional StudiesInvestigates CSTF64’s role in alternative polyadenylation (APA) and cell differentiation .Macrophage differentiation in U937 cells .

Regulatory Role in APA

  • CSTF64 and CSTF64τ exhibit functional redundancy in PAS selection. Codepletion in HeLa cells caused a proximal-to-distal PAS shift in 85% of affected genes, indicating their compensatory roles .

  • CSTF64 binds dormant intronic PASs suppressed by U1 snRNP, preventing premature transcription termination .

Role in Cellular Differentiation

  • Macrophage Differentiation:

    • CSTF64 knockdown (KD) in U937 cells reduced CD68/CD38 (macrophage markers) and increased CD14 (monocyte marker), impairing differentiation .

    • Overexpression (OE) enhanced macrophage marker expression and morphological complexity .

Study ModelInterventionOutcome
U937 cells + PMACSTF64 KDReduced cell attachment and granularity .
U937 cellsCSTF64 OEIncreased CD68/HLA-DRA expression .

Disease Relevance

  • CSTF64 overexpression promotes lung cancer proliferation .

  • Dysregulation linked to neurodegeneration and cardiac fibrosis .

Validation and Specificity

  • Specificity Confirmation: Dual-color Western blotting distinguishes CSTF64 (64 kDa) and CSTF64τ (70 kDa) in human/mouse cells .

  • Functional Validation: RNAi-mediated CSTF64 depletion confirmed antibody specificity via reciprocal CSTF64τ upregulation .

Validation MethodKey Result
Western BlotDistinct bands at expected molecular weights .
shRNA KnockdownReduced CSTF64 protein levels without affecting CSTF64τ mRNA .

Product Specs

Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
Made-to-order (14-16 weeks)
Synonyms
CSTF64 antibody; ESP1 antibody; At1g71800 antibody; F14O23.18Cleavage stimulating factor 64 antibody; AtCstF-64 antibody; AtCstF64 antibody; CF-1 64 kDa subunit antibody; Cleavage stimulation factor 64 kDa subunit antibody; CSTF 64 kDa subunit antibody; Protein ENHANCED SILENCING PHENOTYPE 1 antibody; Protein SUPPRESSORS OF OVEREXPRESSED FCA 19 antibody; SOF19 antibody
Target Names
CSTF64
Uniprot No.

Target Background

Function
CSTF64 Antibody is a crucial component involved in the polyadenylation and 3'-end cleavage of pre-mRNAs. This subunit plays a direct role in binding to pre-mRNAs, particularly within the 3' non-coding region. It is essential for the targeted 3' processing of antisense transcripts, a process that initiates transcriptional silencing of the corresponding sense gene.
Database Links

KEGG: ath:AT1G71800

STRING: 3702.AT1G71800.1

UniGene: At.12026

Subcellular Location
Nucleus.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
© Copyright 2025 TheBiotek. All Rights Reserved.