CSTF64 Antibody detects the CSTF64 protein, which recognizes GU-rich elements downstream of polyadenylation sites (PAS) in pre-mRNAs. CSTF64 is part of the cleavage stimulation factor (CSTF) complex, essential for mRNA 3ʹ-end cleavage and polyadenylation. This process ensures proper mRNA maturation and stability, influencing gene expression regulation.
Applications in Research
CSTF64 Antibody is widely used for:
Application
Description
Examples from Literature
Western Blot (WB)
Detects CSTF64 (~64 kDa) and CSTF64τ (~70 kDa) in human/mouse cell lines.
Validation in HeLa, COLO 320, and mouse tissues.
Immunoprecipitation (IP)
Identifies CSTF64-protein interactions (e.g., with symplekin).
Co-IP studies in HeLa cells.
Immunohistochemistry (IHC)
Localizes CSTF64 in tissues (e.g., breast cancer).
Detection in human tumor samples.
Functional Studies
Investigates CSTF64’s role in alternative polyadenylation (APA) and cell differentiation.
Macrophage differentiation in U937 cells.
Regulatory Role in APA
CSTF64 and CSTF64τ exhibit functional redundancy in PAS selection. Codepletion in HeLa cells caused a proximal-to-distal PAS shift in 85% of affected genes, indicating their compensatory roles.
CSTF64 Antibody is a crucial component involved in the polyadenylation and 3'-end cleavage of pre-mRNAs. This subunit plays a direct role in binding to pre-mRNAs, particularly within the 3' non-coding region. It is essential for the targeted 3' processing of antisense transcripts, a process that initiates transcriptional silencing of the corresponding sense gene.