CTF7 Antibody
Description
Definition and Biological Context
CTF7 (Chromosome Transmission Fidelity 7) is an acetyltransferase critical for sister chromatid cohesion during S phase. Its antibody enables researchers to investigate CTF7's localization, expression patterns, and molecular interactions .
Antibody Development and Validation
The Arabidopsis CTF7 antibody was generated using recombinant full-length CTF7 protein expressed in E. coli. Key validation steps included:
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Specificity: Confirmed via immunolocalization in ctf7 mutant tissues showing reduced signal .
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Applications:
Tissue-Specific Expression
RT-PCR and expression profiling revealed CTF7 transcript levels are highest in actively dividing tissues:
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Bolting plants: Elevated expression during rapid growth phases .
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Bud and young flower tissues: High transcriptional activity .
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Mature leaves: Minimal expression, aligning with reduced mitotic activity .
Table 2: CTF7 Expression Across Arabidopsis Tissues
| Tissue | Relative CTF7 Transcript Level |
|---|---|
| Buds | High |
| Young seedlings | Moderate |
| Mature leaves | Low |
Subcellular Localization
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Embryo: Strong nuclear signal, with weaker cytoplasmic staining .
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Endosperm: Barely detectable levels, suggesting tissue-specific regulation .
Functional Insights from Antibody Studies
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Role in Mitosis: CTF7 antibody staining confirmed its necessity for mitotic progression in Arabidopsis female gametophytes. Mutant ctf7 gametophytes showed delayed mitosis and abnormal antipodal cell degeneration .
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Interaction with DNA Replication Machinery: Genetic interactions between CTF7, POL30 (PCNA), and CTF18 were identified, linking cohesion establishment to DNA replication .
Technical Limitations and Advancements
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- CTF7 influences cytosine methylation, particularly symmetric CG methylation, and the expression of the copia28 retrotransposon. PMID: 26039473
- Elevated expression of AtCTF7 mutant proteins disrupts megasporogenesis, megagametogenesis, and male meiosis, leading to a broad range of vegetative defects, including dwarfism, which are inherited in a non-Mendelian pattern. PMID: 25848842
- Research has shown that mutations in AtWAPL suppress the lethality associated with the inactivation of AtCTF7. PMID: 25033056
- Arabidopsis CTF7/ECO1 plays crucial roles in preserving genomic integrity and meiosis. PMID: 23750584
- CTF7 is essential for the establishment of sister chromatid cohesion and the association of cohesin with chromatin during meiosis. PMID: 23941555
- Seeds containing T-DNA insertions in CTF7 exhibit embryo lethality early in development, while the endosperm develops normally. Overexpression of CTF7 inhibits female gametophyte development. PMID: 20671110
