2C Antibody

Overview of 2C11-2 Antibody

The 2C11-2 monoclonal antibody (Mab) is a mouse-derived IgG1 isotype antibody generated against Drosophila melanogaster Dynein heavy chain (Dhc), a critical motor protein involved in intracellular transport and mitosis .

Key Properties

Antibody Structure

As a typical immunoglobulin, 2C11-2 features:

• Y-shaped tetramer: Composed of two heavy (H) and two light (L) chains .

• Variable (Fab) region: Binds specifically to Dhc epitopes.

• Constant (Fc) region: Mediates immune effector functions (e.g., binding to Fc receptors) .

Target Biology

Dynein heavy chain is essential for:

• Retrograde transport along microtubules.

• Mitotic spindle organization.

• Ciliary and flagellar motility .

Experimental Use Cases

• Functional Blocking: Inhibits Dynein activity in Drosophila models to study mitotic defects .

• Immunofluorescence: Localizes Dhc in cellular structures (e.g., mitotic spindles).

• Western Blot: Detects Dhc (~530 kDa) in protein extracts .

Validation Data

• Specificity: Recognizes Dhc with minimal off-target binding in Drosophila lysates .

• Solvent Tolerance: Compatible with buffers containing detergents (e.g., Triton X-100) .

Comparison to Related Antibodies

While 2C11-2 targets structural proteins, other "2C"-designated antibodies (e.g., clone 2-13 in trichothecene toxin detection ) serve distinct roles:

Limitations and Future Directions

• Species Restriction: Limited to Drosophila unless cross-reactivity with orthologs is confirmed.

• Therapeutic Potential: Not currently used in clinical settings, unlike FDA-approved antibodies targeting human proteins .