C/VIF2 Antibody
Description
Definition and Target Specificity
The C/VIF2 Antibody is a polyclonal antibody produced against the cell wall/vacuolar inhibitor of fructosidase 2 (C/VIF2) protein, encoded by the At5g64620 gene in Arabidopsis thaliana. It specifically binds to C/VIF2, a regulatory protein that inhibits the activity of fructosidases (CWI and VI) in plant cells.
Mechanism of Action
C/VIF2 Antibody operates by neutralizing the C/VIF2 protein, thereby reducing its inhibitory effect on fructosidases. This mechanism has downstream implications for sucrose metabolism and energy distribution in plants:
| Fructosidase Type | Location | Role in Plants |
|---|---|---|
| Cell wall invertase | Cell wall | Facilitates sucrose unloading for growth |
| Vacuolar invertase | Vacuole | Regulates osmotic balance and stress response |
By modulating these enzymes, the antibody enables researchers to study carbohydrate allocation during developmental stages or environmental stressors.
A. Functional Studies
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Sucrose Metabolism: Used to investigate how C/VIF2-mediated inhibition of fructosidases affects plant growth and yield.
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Stress Response: Enables analysis of carbohydrate redistribution during drought or pathogen attack.
B. Agricultural Biotechnology
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Crop Optimization: Potential utility in engineering plants with altered sucrose partitioning for improved stress tolerance or biomass production.
Comparison to Other Antibody Types
While monoclonal antibodies (mAbs) like those against CSFV E2/E rns or scFv fragments are engineered for high specificity or therapeutic use, the C/VIF2 Antibody is a polyclonal reagent optimized for broad epitope recognition in plant studies.
| Feature | C/VIF2 Antibody | scFv/mAb |
|---|---|---|
| Target | Plant fructosidase inhibitor | Pathogens, viral antigens |
| Production Method | Polyclonal (rabbit host) | Monoclonal/phage display |
| Primary Use | Research | Diagnostics/therapeutics |
Future Directions
Product Specs
Composition: 50% Glycerol, 0.01M PBS, pH 7.4
