cwc-15 Antibody

CWC15 Protein Overview

CWC15 is a conserved eukaryotic protein encoded by the CWC15 gene located on human chromosome 11q21 (NCBI Gene ID: 51503) . Key features include:

CWC15 stabilizes spliceosomal components and interacts with miRNA-processing proteins like HYL1 and SERRATE (SE) to regulate pri-miRNA transcription and processing .

CWC15 Antibody Characteristics

Commercial CWC15 antibodies are primarily monoclonal or polyclonal reagents validated for diverse applications:

Research Applications

CWC15 antibodies are utilized in multiple experimental workflows:

Role in miRNA Biogenesis

• Mechanism: CWC15 binds MIR gene promoters to enhance RNA Polymerase II occupancy, boosting pri-miRNA transcription. It also stabilizes HYL1-pri-miRNA interactions, improving processing efficiency .

• Regulation: CWC15 promotes phosphorylation and proteasomal degradation of SERRATE (SE), preventing excessive SE accumulation that disrupts miRNA maturation .

Disease Associations

• Neurological Disorders: CWC15 is linked to ADHD and conduct disorder, though mechanistic insights remain under investigation .

• Developmental Defects: CWC15 knockdown in Arabidopsis causes growth abnormalities and embryo lethality, underscoring its essential role .

Technical Considerations

• Storage: Antibodies are typically supplied in PBS with 0.1% sodium azide and require storage at 4°C .

• Controls: Blocking peptides (e.g., sc-390450 P) are available for competition assays .

Future Directions

Current studies focus on:

1. Elucidating CWC15’s role in viral RNA processing and COVID-19 pathogenesis .

2. Developing therapeutic strategies targeting CWC15-protein interactions in neurological disorders .