48 kDa cell wall Antibody

Cell Wall Development and Somatic Embryogenesis

In Dactylis glomerata, the 48 kDa protein EP48 is transiently expressed during early somatic embryogenesis. MAb 3G2, which binds EP48, serves as an early marker for embryogenic potential in suspension cultures . Key findings include:

• Localization: EP48 accumulates in cell walls of microclusters and proembryogenic masses but not globular embryos .

• Marker Utility: Detection of EP48 precedes morphological changes in embryogenesis, enabling early identification of embryogenic cell lines .

Detection and Quantification

Antibodies against the 48 kDa cell wall protein enable precise detection via:

For Arabidopsis, pre-validated antibody combinations (e.g., X-P80848-N, X-P80848-C) simplify detection workflows .

Comparative Analysis of Antibody Combinations

The following table compares antibody combinations for Arabidopsis 48 kDa cell wall protein detection:

These combinations are designed for multiplex assays, with deconvolution services available for epitope mapping ($100 per combination) .

Cell Wall Dynamics

Plant cell walls are dynamic structures composed of polysaccharides, proteoglycans, and polyphenolics. The 48 kDa protein may interact with these components, as antibodies targeting cell wall glycans (e.g., pectins, hemicelluloses) are widely used to study structural modifications .

Future Directions

• Functional Studies: Investigating the role of the 48 kDa protein in stress responses or cell wall remodeling.

• Multi-Omics Integration: Combining antibody-based detection with transcriptomics/proteomics to map cell wall protein networks .

• Agricultural Applications: Developing markers for embryogenesis in crop species to improve tissue culture protocols .

References

1. Frontiers in Plant Science (2018) – Antibody toolbox for plant cell wall analysis .

2. Ab-mart – Antibody combinations for Arabidopsis 48 kDa cell wall protein .

3. PubMed – EP48 as a marker for somatic embryogenesis .