CYTB5-E Antibody

Overview of Cytochrome b5 and Its Isoforms

Cytochrome b5 exists in multiple isoforms across species. In Arabidopsis thaliana, five isoforms (AtCB5-A to AtCB5-E) have been identified, with AtCB5-E (At5g53560) being one of the least characterized . These isoforms share conserved features:

• A cytosolic heme-binding domain with the -HPGG- motif.

• A transmembrane domain anchoring the protein to membranes (ER, mitochondria, or chloroplasts).

• Roles in electron transfer for fatty acid desaturation, lignin biosynthesis, and redox signaling .

Antibodies Targeting Cytochrome b5

While no commercial antibody explicitly named "CYTB5-E" is documented, several antibodies target cytochrome b5 broadly. Key examples include:

Key Applications

• Cancer Research:

  • CYB5A overexpression correlates with altered lipid profiles in hepatocellular carcinoma .

  • Anti-CYB5 antibodies detect reduced staining in lung epithelial cells during inflammation .

• Metabolic Studies:

  • CYB5R3-overexpressing mice show improved mitochondrial function and delayed age-related pathologies .

  • Hepatic CYB5 knockout models reveal substrate-dependent effects on drug clearance .

Technical Validation

• Western Blot: Anti-CYB5 antibodies (e.g., ab69801) show a 15 kDa band in human liver extracts .

• Immunohistochemistry: Strong CYB5 expression in bronchiolar epithelium decreases during lung injury .

Gaps and Opportunities

• Plant-Specific CYB5 Isoforms: Despite Arabidopsis AtCB5-E's role in lignin biosynthesis , no isoform-specific antibodies are commercially available.

• Therapeutic Potential: CYB5R activators (e.g., tetrahydroindenoindole) extend lifespan in models by enhancing oxidative stress resistance .