Cyclophilin B Antibody
Description
Types and Specificity
Cyclophilin B antibodies are available as monoclonal or polyclonal variants, targeting epitopes in the C-terminal region or full-length protein. Key features include:
| Antibody Type | Host | Specificity | Applications |
|---|---|---|---|
| Monoclonal (e.g., MAB5410) | Mouse | Human, mouse, rat | WB, IF/ICC, IHC |
| Polyclonal (e.g., AF5410) | Goat/Rabbit | Human, mouse, rat | WB, IHC, IF/ICC, ELISA |
These antibodies are validated for detecting CypB in endoplasmic reticulum (ER) and secreted forms, with no cross-reactivity to cyclophilin A or other family members .
Applications in Research
Cyclophilin B antibodies are employed in diverse methodologies:
Western Blotting (WB)
Immunohistochemistry (IHC)
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Tissue Staining: Human liver, colon, and lymphoma tissues (antigen retrieval with TE buffer pH 9.0) .
Immunofluorescence (IF/ICC)
ELISA and Knockout Validation
Cross-Reactivity and Species Specificity
| Species | Reactivity | Cell/Tissue Models |
|---|---|---|
| Human | Strong | HeLa, A431, U-937, Raji, HepG2 . |
| Mouse | Moderate | NIH/3T3, BaF3, RAW 264.7 . |
| Rat | Moderate | Rat liver, NRK, Y3-Ag . |
Note: Cross-reactivity with other species (e.g., non-human primates) requires empirical testing .
Metabolic Syndrome (MetS)
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Elevated serum CypB levels correlate with MetS severity, suggesting its role as a biomarker . Antibodies are used to quantify CypB in patient cohorts.
HIV-1 Infection
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Overexpression of intracellular CypB enhances HIV nuclear DNA import, detected via WB in transfected cells .
Malaria
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Anti-CypB antibodies reveal its surface expression on red blood cells (RBCs), critical for Plasmodium falciparum invasion .
Autoimmune Diseases
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High CypB levels in rheumatoid arthritis and lupus sera indicate its proinflammatory role, detectable via ELISA .
Data Tables
Table 1: Antibody Dilutions for Common Applications
Table 2: Cross-Reactivity and Cell Lines
Product Specs
Product Science Overview
Cyclophilin B is involved in several cellular processes, including:
- Protein Folding: It assists in the proper folding of newly synthesized proteins within the ER.
- Immune Response: Cyclophilin B is secreted in response to inflammatory stimuli and interacts with CD147, a receptor involved in immune cell activation .
- Calcium Signaling: The CyPB-cyclosporin A complex inhibits calcineurin, a calcium and calmodulin-dependent protein phosphatase, which is crucial for T-cell activation .
The Mouse Anti-Human Cyclophilin B Antibody is a monoclonal antibody that specifically targets human Cyclophilin B. This antibody is widely used in various research applications, including:
- Western Blotting (WB): To detect Cyclophilin B in cell lysates .
- Immunohistochemistry (IHC): For staining tissue sections to study the localization of Cyclophilin B .
- Immunocytochemistry (ICC): To visualize Cyclophilin B in cultured cells .
- Flow Cytometry (FC): For analyzing Cyclophilin B expression in different cell populations .
- Enzyme-Linked Immunosorbent Assay (ELISA): To quantify Cyclophilin B levels in various samples .
Cyclophilin B and its antibodies are valuable tools in biomedical research. They help in understanding the role of Cyclophilin B in protein folding, immune response, and calcium signaling. Additionally, studying Cyclophilin B can provide insights into various diseases, including cancer and inflammatory disorders.
For more detailed information, you can refer to the Proteintech and R&D Systems websites.
