cyk-7 Antibody

Overview of CK7 Antibody

CK7 antibodies are monoclonal immunoglobulins designed to detect Cytokeratin 7, a 55 kDa protein encoded by the KRT7 gene. These antibodies are widely used to identify epithelial origin in tumors and study cellular differentiation .

Key Features:

• Host Species: Rabbit (e.g., clone EPR17078) or mouse (e.g., clones OV-TL 12/30, KRT7/760 + KRT7/903) .

• Specificity: Validated using knockout cell lines and peptide absorption .

• Applications: Western blot (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), flow cytometry, and immunofluorescence (IF) .

Applications and Protocols

CK7 antibodies are optimized for diverse methodologies:

Performance Notes:

• The BC1 rabbit monoclonal antibody demonstrates superior intensity and debris staining compared to the mouse OV-TL 12/30 clone .

• Recombinant formats ensure batch-to-batch consistency .

Clinical and Diagnostic Utility

CK7 expression patterns aid in tumor classification and prognosis:

Tumor Differentiation

Mechanistic Insights

• CK7 regulates HPV16 E7 mRNA translation and interferon-dependent cell cycle arrest .

• In CRC, CK7+ tumors exhibit dedifferentiation markers and aggressive budding phenotypes .

Technical Advancements

• Multiplex Staining: CK7/CDX2/TTF1 cocktails enable simultaneous detection of multiple biomarkers in lung and gastrointestinal cancers .

• Cross-Reactivity: Rabbit monoclonal antibodies show broader species reactivity (human, mouse, rat) vs. mouse clones (human-specific) .

Validation and Quality Control

• Specificity Checks: Knockout cell lines (e.g., KRT7–/–) confirm absence of off-target binding .

• Reproducibility: Affinity purification via Protein A/G ensures >90% purity .

Limitations and Considerations

• False Negatives: Prolonged formalin fixation may mask epitopes; antigen retrieval is critical .

• Contextual Interpretation: CK7 must be paired with site-specific markers (e.g., TTF1 for lung) to avoid misdiagnosis .