cyn-2 Antibody

Chemical and Optical Properties

Cy2 conjugates exhibit maximum excitation at 492 nm and emission at 510 nm, similar to FITC but with enhanced photostability and reduced pH sensitivity . Key comparisons with other green fluorophores:

Immunofluorescence Staining

• Dilution Range: 1:50 – 1:200 for most applications, depending on antigen density and permeability .

• Cross-Reactivity: Minimal cross-reactivity with human, bovine, horse, mouse, and rabbit serum proteins ensures specificity in multiplex assays .

• Comparative Performance:

  • Advantages: Superior brightness in plastic media compared to FITC and DyLight 488 .

  • Disadvantages: Outperformed by Alexa Fluor® 488 in aqueous media due to higher photostability and brightness .

Diagnostic Limitations

Cy2-conjugated antibodies are labeled for in vitro research use only and are not approved for diagnostic or therapeutic purposes .

Mounting Media Compatibility

Case Study: Cy2 in Antibody-Fluorophore Conjugate Research

A systematic evaluation of functional groups on antibody-fluorophore conjugates highlighted Cy2’s utility in targeted imaging :

• Tumor Targeting: Zwitterionic and pegylated Cy2 derivatives demonstrated high tumor-to-background ratios (TBR) in preclinical models.

• Off-Target Uptake: Cationic Cy2 variants showed elevated liver uptake, while hydrophobic derivatives exhibited nonspecific Kupffer cell binding .