CYP51A1 Antibody
Description
Definition and Overview
The CYP51A1 antibody is a polyclonal rabbit antibody designed to target the cytochrome P450, family 51, subfamily A, polypeptide 1 (CYP51A1) protein. CYP51A1 is a key enzyme in the cholesterol biosynthesis pathway, specifically catalyzing the removal of the 14α-methyl group from lanosterol . This antibody is widely used in biomedical research for detecting and studying CYP51A1 expression in various applications, including Western blot (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA) .
Key Features:
-
Reactivity: Validated for human, mouse, rat, chicken, hamster, and other species .
-
Immunogen: Recombinant fusion protein corresponding to amino acids 245–420 of CYP51A1 .
-
Format: Unconjugated liquid antibody stored in PBS with sodium azide and glycerol .
Tested Applications
Recommended Dilutions
Role in Pancreatic Cancer Resistance
Recent studies in Nature (2025) identified CYP51A1 as a critical regulator of pH-dependent cell death in pancreatic ductal adenocarcinoma (PDAC) . The antibody was used to validate CYP51A1 knockdown in cell lines (SW1990, MIAPaCa2) and xenograft models. Key findings:
-
Mechanism: CYP51A1 prevents lysosomal proton efflux mediated by TMEM175, thereby inhibiting alkalization-induced cell death triggered by the drug JTC801 .
-
Therapeutic Implications: Knockdown or pharmacological inhibition of CYP51A1 enhances JTC801 efficacy in tumor suppression .
Prognostic Value
CYP51A1 expression correlates with poor prognosis in cancers such as cervical squamous cell carcinoma and kidney chromophobe carcinoma . Antibody-based detection has been used to validate these associations in clinical samples .
Cancer Biomarker
CYP51A1 serves as a prognostic marker in multiple cancers, including:
-
Cervical squamous cell carcinoma (high expression linked to worse outcomes) .
-
Head and neck squamous cell carcinoma (marker of aggressive disease) .
-
Kidney chromophobe and clear cell carcinoma (disease progression) .
Metabolic Regulation
The antibody has been used to study CYP51A1’s role in cholesterol homeostasis and its degradation under inflammatory conditions (via nitric oxide signaling) .
Antibody Characteristics
Product Specs
Target Background
- This research investigates the regulation of human CYP51A1 expression, the lanosterol 14alpha-demethylase. PMID: 28830911
- This study provides molecular insights into the substrate profile, enhanced catalysis, and reduced susceptibility to inhibition of human CYP51. PMID: 27313059
- This research suggests that GDF9, possibly in conjunction with FSH, may play significant roles in regulating cholesterol biosynthesis and the expression of CYP51A1 in granulosa cells. This could potentially serve as a predictor for unfertilization. PMID: 24711211
- This study demonstrates that forkhead transcription factor 4 (FoxO4) interacts with sterol regulatory element binding protein (SREBP)2 and hypoxia inducible factor (HIF)2alpha to modulate lanosterol 14alpha demethylase (CYP51) promoter activity. PMID: 24353279
- The findings of this research indicate a novel connection between the cholesterol synthesis gene CYP51A1 and pregnancy complications. PMID: 24358204
- This research investigated the selective interaction of azoles with human cytochrome P450 51A1, revealing the highest affinity for ketoconazole. PMID: 24502137
- This research observed low nucleotide variability of CYP51A1 within cholesterol and bile acid synthesis and xenobiotic metabolism pathways. PMID: 24362992
- This study analyzed the impact of the novel CYP51A1 inhibitor 2-((3,4-dichlorophenethyl)(propyl)amino)-1-(pyridin-3-yl)ethanol (LEK-935) on the proteome of primary human hepatocytes. PMID: 22180046
- This research indicates that CPY51 structures from various eukaryotic organisms exhibit striking similarities. PMID: 20547249
- This study observed substantial conformational changes in the B' helix and F-G loop regions upon ligand binding, consistent with the membrane nature of the protein and its substrate. PMID: 20149798
- This research revealed that a cAMP-responsive element binding site is essential for sterol regulation of the human lanosterol 14alpha-demethylase gene. PMID: 12145339
- This research highlights the primary roles of specific regions in determining the strength of interactions with azoles. PMID: 15611056
- This study demonstrates that the liver X receptor alpha directly silences the expression of two key cholesterologenic enzymes (lanosterol 14alpha-demethylase (CYP51A1), and squalene synthase (farnesyl diphosphate farnesyl transferase 1)) via novel negative LXR DNA response elements. PMID: 18676367
