cyn-7 Antibody

Applications in Research and Diagnostics

Cy7-conjugated antibodies are critical tools in:

Flow Cytometry

• Detection of surface markers (e.g., CD3, CD14, CD19) with high sensitivity due to minimal spectral overlap .

• Example: PE/Cyanine7 anti-human CD14 (Clone 61D3) shows >90% staining efficiency in human peripheral blood mononuclear cells .

In Vivo Imaging

• Enabled by Cy7’s deep tissue penetration (up to 3 cm) for tracking antibody distribution .

Immunohistochemistry

• Used in paraffin-embedded and frozen sections for subcellular localization studies (e.g., cytokeratin 7 detection in bile duct epithelia ).

T-Cell Acute Lymphoblastic Leukemia (T-ALL)

• Anti-CD7 antibodies conjugated to Cy7 analogs (e.g., J87-Dxd ADC) showed:

  • IC50 values of 6.3 nM against CCRF-CEM cells .

  • Rapid internalization into CD7+ leukemia cells .

HIV-1 Prevention

• VRC07-523LS (a CD4-binding site antibody) engineered with extended half-life (42 days vs. 15 days for VRC01) used Cy7 analogs for pharmacokinetic tracking .

Mitochondrial Studies

• Cytochrome c antibodies (Clone 7H8.2C12) conjugated to biotin/Cy7 detected 15 kDa bands in HeLa lysates, confirming mitochondrial localization .

Stability and Handling

• Storage: 2–8°C; avoid freezing .

• Shelf life: 12–18 months when protected from light .

• Bioavailability: Intravenous administration yields higher serum concentrations than subcutaneous routes (e.g., 20 mg/kg IV vs. 5 mg/kg SC ).

Limitations and Challenges

• Cross-reactivity: Some clones (e.g., anti-CD3) show mitogenic effects on T cells .

• Signal interference: Residual APC emission in Cy7 tandems requires compensation controls .