dact2 Antibody

Introduction to DACT2 Antibody

The DACT2 antibody is a specialized immunological tool targeting the Dishevelled-binding antagonist of β-catenin 2 (DACT2) protein, a regulator of Wnt/β-catenin and TGF-β signaling pathways. This antibody enables researchers to study DACT2's roles in embryonic development, cancer biology, and cardiovascular diseases. Validated across diverse applications, it is critical for detecting DACT2 expression, localization, and functional interactions in cellular and tissue contexts .

Cancer Biology

• Tumor Suppression:

  • DACT2 antibodies identified reduced DACT2 expression in esophageal squamous cell carcinoma (ESCC) via immunohistochemistry (1:100 dilution; Abcam ab79042), correlating with poor differentiation and survival .

  • In nasopharyngeal carcinoma (NPC), DACT2 overexpression inhibited cell migration/invasion (via Transwell assays) and reduced MMP2/9 expression .

  • Breast cancer studies revealed DACT2 methylation-associated silencing, with antibody-based WB confirming re-expression suppressed tumor growth and metastasis .

• Mechanistic Insights:

  • DACT2 interacts with β-catenin to disrupt Wnt signaling, as shown by co-immunoprecipitation (Co-IP) and GST pull-down assays .

  • In lung cancer, DACT2 antibody-based IHC linked promoter hypermethylation to poor prognosis and β-catenin accumulation .

Cardiovascular Research

• Atrial Fibrillation (AF):

  • DACT2 antibody (Abcam ab79042; 1:100) revealed reduced atrial fibrosis in AF patients. Strong DACT2 expression inversely correlated with collagen I/III levels .

  • Overexpression studies showed DACT2 modulates KCNE5, KCNH2, and SCN5A ion channels, influencing electrical remodeling .

Table 1: DACT2 Antibody Performance in Select Studies

Technical Considerations

• Storage: Store at −20°C (Sigma HPA030251) or +4°C (Abcam ab79042) .

• Controls: Include antigen retrieval (citrate buffer, pH 6.0) and IgG-negative controls to ensure specificity .

• Limitations: Cross-reactivity with DACT1 may occur due to structural homology; validate using knockdown models .