daf-19 Antibody
Description
What is the DAF-19 Antibody?
The DAF-19 antibody refers to polyclonal antibodies generated to detect specific isoforms of the DAF-19 transcription factor. Two primary antibodies were developed:
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AbDAF19N: Targets the N-terminal region (amino acids 2–212) and detects a 120 kDa band on Western blots, corresponding to DAF-19A/B isoforms .
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AbDAF19C: Targets the C-terminal region (amino acids 340–513) but is unsuitable for Western blotting .
Both antibodies localize DAF-19 to neuronal nuclei and hypodermal cells in C. elegans, aiding in the study of its developmental and immune functions .
2.1. Role in Innate Immunity
DAF-19 collaborates with ATF-7 (a CREB/ATF-family transcription factor) to regulate:
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Serotonin biosynthesis: Upregulates tph-1 in ADF chemosensory neurons during Pseudomonas aeruginosa infection .
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Antimicrobial gene expression: Controls intestinal immune responses (e.g., T24B8.5, K08D8.5) against pathogens .
Key Data:
2.2. Isoform-Specific Functions
DAF-19 has three isoforms with distinct roles:
Antibody Utility:
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AbDAF19N confirmed isoform-specific expression patterns via immunostaining .
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AbDAF19C revealed hypodermal expression during larval stages .
3.1. Western Blotting
3.2. Immunostaining
Clinical and Evolutionary Insights
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Evolutionary conservation: DAF-19’s interaction with ATF-7 mirrors RFX-CREB collaboration in human immune cells, suggesting ancient immune regulatory mechanisms .
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Therapeutic potential: RFX transcription factors are linked to human ciliopathies, making DAF-19 a model for studying congenital disorders .
Table 1: Survival Rates on PA14
Table 2: Antimicrobial Gene Expression
Product Specs
Target Background
DAF-19 is a transcription factor potentially regulating gene expression in ciliated sensory neurons. While it may activate expression of shared components in sensory cilia, it does not appear to regulate cell-type-specific expression. In conjunction with the transcription factor ATF-7, DAF-19 is involved in modulating the serotonergic response of ADF neurons to pathogenic food sources.
DAF-19 Function and Related Studies:
- Aberrant splicing of daf-19c to upstream cryptic sites produces functional, in-frame products. (PMID: 25637722)
- Pathogenic cues activate a core signaling pathway through tissue-specific mechanisms, highlighting a novel role for RFX factors in neuronal and innate immune responses to infection. (PMID: 23505381)
- Evidence suggests that an enhancer element co-regulates ciliary genes in a DAF-19-dependent manner. (PMID: 22683808)
- Transcriptional profiling of C. elegans DAF-19 identified a ciliary base-associated protein and a CDK/CCRK/LF2p-related kinase crucial for intraflagellar transport. (PMID: 21740898)
- The RFX transcription factor DAF-19 regulates ciliogenesis through X-box containing ciliogenic genes and controls ciliary specialization by regulating non-X-box containing sensory genes. (PMID: 20923979)
- daf-19 mutations eliminate all sensory cilia, resulting in a complete loss of external sensory input. (PMID: 19652182)
- HOB neuron sensory function requires the combined action of EGL-46 and DAF-19 regulatory pathways. (PMID: 12954713)
- Three distinct functional isoforms of DAF-19 have been characterized. (PMID: 18843046)
