Customize daf-37 Antibody

Product Specs

Buffer

Preservative: 0.03% ProClin 300
Components: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4

Form

Liquid

Lead Time

14-16 Week Lead Time (Made-to-Order)

Synonyms

daf-37 antibody; C30B5.5G-protein coupled receptor daf-37 antibody; Abnormal dauer formation protein 37 antibody

Target Names

daf-37

Uniprot No.

Q18321

Target Background

Function

The daf-37 antibody targets a G-protein coupled receptor (GPCR) that forms a heterodimer with daf-38, regulating dauer formation and behavior. It serves as a receptor for the ascaroside pheromone ascr#2 and is essential for responding to dauer-inducing pheromones, particularly ascr#2. daf-37 exhibits neuron-specific functions in response to ascr#2. In ASI neurons, it controls dauer formation in larvae, while in ASK neurons, it influences longevity and hermaphrodite repulsion. It is believed to function upstream of the daf-11, daf-7, and daf-2 signaling pathways.

Database Links

KEGG: cel:CELE_C30B5.5

Protein Families

G-protein coupled receptor 1 family

Subcellular Location

Cell membrane; Multi-pass membrane protein. Cell projection, cilium. Golgi apparatus.

Tissue Specificity

Expressed in the ASI and ASK chemosensory neurons, in the IL-2 interneurons, and in the male specific CEM (cephalic sensilla, male) neurons.

Q&A

Based on the provided search results and analysis of available scientific literature, here is a structured FAQ collection addressing key research considerations for antibody-related studies in immunosenescence and receptor interaction research:

How to validate antibody specificity for immune senescence biomarkers in aging studies?

Perform multiplexed validation using:
- Flow cytometry for immune cell subset frequencies (e.g., CD4+/CD8+ ratios)
- Cytokine response profiling via Luminex arrays (IL-6, TNF-α, IFN-γ)
- Competitive inhibition assays with recombinant DAF SCR domains
Critical controls:
- Isotype-matched negative controls
- Knockout cell lines for target biomarkers

What methodological considerations apply to antibody-based detection of complement regulatory proteins?

Parameter	DAF (CD55) Analysis	Technical Considerations
Epitope	SCR domains 2-4	Confirm domain specificity via truncated protein variants
Assay	CryoEM binding studies	Low receptor occupancy requires ≥15% density threshold
Cross-reactivity	Echovirus co-receptors	Validate with viral plaque inhibition assays

How to resolve contradictory data in antibody-mediated receptor activation studies?

Case Example: Discrepancies in ErbB2 phosphorylation reports

Experimental redesign:
- Compare antibody clones (e.g., 4D5 vs. MGR3) in isogenic cell lines
- Use single-molecule localization microscopy to quantify receptor clustering
- Perform phosphoproteomic time-course analyses (0-60 min post-treatment)

Key Finding:

"DAF SCR2-3 domains show 65% plaque inhibition efficiency compared to SCR1-4 (85%), indicating domain-dependent functional impacts" .

What integrated approaches address low-affinity antibody interactions in structural studies?

Combine methodologies:
- Hydrogen-deuterium exchange mass spectrometry (HX-MS) for epitope mapping
- Negative-stain EM screening prior to cryoEM (enhances particle selection)
- Surface plasmon resonance with koff analysis (10-3 s-1 threshold)

How to improve antibody fragments for diagnostic applications?

Developability enhancement strategies:
- Framework mutagenesis (e.g., [28D]-FR1-H mutation reduces aggregation)
- Multimodal chromatography polishing (HCP reduction to <50 ppm)
- Camelid VHH engineering for improved thermal stability (ΔTm +8°C)

What orthogonal techniques validate antibody-mediated signaling modulation?

Technique	Application	Key Metrics
Microscale thermophoresis	Binding affinity	K<sub>D</sub> ≤ 100 nM
RNA-Seq	Pathway activation	≥2-fold change in NF-κB targets
Crystallography	Structural validation	3Å resolution threshold

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daf-37 Antibody