daf-9 Antibody
Description
Clarification of Terminology
The designation "daf-9" refers to distinct entities depending on the biological context:
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In C. elegans:
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DAF-9 is a cytochrome P450 enzyme (CYP450) critical for synthesizing dafachronic acid (DA), a steroid hormone that regulates larval development, lifespan, and germline proliferation via the nuclear receptor DAF-12 .
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No commercial antibodies targeting C. elegans DAF-9 are documented in the provided sources.
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In Human Biology:
Key Findings from Research:
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Genetic Interactions:
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Mechanistic Insights:
Table 1: Key Genetic and Functional Data for C. elegans DAF-9
Human CD55/DAF Antibodies
Available antibodies target human CD55/DAF, a glycoprotein regulating complement activation. These are unrelated to C. elegans DAF-9 but are included here due to nomenclature overlap:
Critical Analysis of "daf-9 Antibody"
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No peer-reviewed studies or commercial products describe antibodies specific to C. elegans DAF-9.
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The conflation likely arises from abbreviating "DAF" (decay-accelerating factor) in human biology with C. elegans gene names (e.g., daf-9).
Recommendations for Researchers
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For C. elegans studies: Use genetic tools (e.g., daf-9 mutants, RNAi) to investigate DAF-9 function.
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For human CD55 studies: Employ validated antibodies (e.g., AF2009, #38730) with proper controls.
Product Specs
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Studies have demonstrated that the expression of DAF-9 is upregulated in *C. elegans* subjected to dietary restriction. PMID: 25209682
- Research suggests that ncr-1 and ncr-2 are involved in intracellular cholesterol processing in *C. elegans*. The Daf-c phenotype observed in the ncr-2; ncr-1 mutant is attributed to a sterol-signaling defect. PMID: 15509773
- Based on experimental findings, it has been proposed that DAF-9 produces a hormone, which acts through DAF-12, to rescue the dauer phenotype. PMID: 15569358
- Two distinct 3-keto-cholestenoic acid metabolites of DAF-9, acting as ligands for DAF-12, have been identified, highlighting their importance in signaling pathways regulated by this enzyme. PMID: 16529801
