DBN1 Antibody
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Description
Overview of DBN1 Antibody
The DBN1 antibody is a specific immunoglobulin designed to detect Drebrin 1 (DBN1), an actin-binding protein encoded by the DBN1 gene on human chromosome 5q35.3. This protein plays critical roles in neuronal development, synaptic plasticity, and immune cell function . The antibody is widely used in research to study DBN1 expression in diseases such as Alzheimer’s disease, breast cancer, and viral infections .
Key Features
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Target: Drebrin 1 (DBN1), an actin-binding protein with isoforms Drebrin A (neuronal) and Drebrin E (non-neuronal) .
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Applications: Western blotting (WB), immunohistochemistry (IHC), immunofluorescence (IF), and immunoprecipitation (IP) .
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Species Reactivity: Primarily human, with cross-reactivity in mouse and rat .
Applications of DBN1 Antibody
Breast Cancer Biomarker
DBN1 expression has been identified as a prognostic marker in luminal breast cancer. High DBN1 levels correlate with aggressive tumor characteristics and poor response to endocrine therapy . Studies using DBN1 antibodies (e.g., Abcam ab268046) demonstrated that elevated DBN1 predicts recurrence and metastasis in treated patients .
Immune Cell Function
In dendritic cells, DBN1 regulates antigen presentation and T-cell activation by stabilizing actin filaments . Antibodies like Cell Signaling’s 96540 revealed impaired phagocytosis and reduced surface receptor expression in DBN1-silenced cells .
Viral Entry Restriction
DBN1 inhibits dynamin-mediated endocytosis, restricting rotavirus and other viral entries. Antibodies (e.g., Proteintech 10260-1-AP) confirmed DBN1’s interaction with cortactin at actin filaments, critical for this antiviral mechanism .
Glaucoma Diagnostics
Circulating DBN1 levels correlate with retinal ganglion cell injury in glaucoma patients, suggesting its potential as a biomarker for disease severity .
Challenges and Considerations
Product Specs
Buffer
Phosphate-buffered saline (PBS) with 0.1% sodium azide, 50% glycerol, pH 7.3. Store at -20°C. Avoid repeated freeze-thaw cycles.
Lead Time
Typically, we can ship the products within 1-3 business days after receiving your orders. Delivery time may vary depending on the purchase method or location. Please consult your local distributors for specific delivery timeframes.
Synonyms
D0S117E antibody; DBN 1 antibody; DBN1 antibody; Developmentally regulated brain protein antibody; Developmentally-regulated brain protein antibody; DKFZp434D064 antibody; DREB_HUMAN antibody; Drebrin 1 antibody; Drebrin antibody; Drebrin E antibody; Drebrin E2 antibody; Drebrin1 antibody; DrebrinE antibody
Target Names
DBN1
Uniprot No.
Target Background
Function
Drebrin is an actin cytoskeleton-organizing protein that plays a crucial role in the formation of cell projections. It is essential for actin polymerization at immunological synapses (IS) and for the recruitment of the chemokine receptor CXCR4 to IS. Drebrin also plays a significant role in dendritic spine morphogenesis and organization, including the localization of the dopamine receptor DRD1 to the dendritic spines. It is involved in memory-related synaptic plasticity in the hippocampus.
Gene References Into Functions
- Drebrin might regulate the activities of the epigenetic reader ZMYND8 through its cytoplasmic sequestration. PMID: 28966017
- Inhibition of cortactin or dynamin-2 abolished the increased virus entry observed in DBN1-deficient cells, suggesting that DBN1 suppresses dynamin-mediated endocytosis via interaction with cortactin. PMID: 28416666
- The absence of drebrin leads to dysfunction of cell-cell communication, resulting in aberrant migration of metastatic cancer cells, impaired synaptic function in dementia, and rupture of endothelial integrity. PMID: 28865011
- One potential pathway coupling actin filaments to microtubules involves the actin filament-binding protein drebrin and the microtubule-binding +TIP protein EB3. This pathway is regulated proximally by cyclin-dependent kinase 5 phosphorylation of drebrin, but the upstream elements in the pathway remain unidentified. PMID: 28865014
- These findings indicate that drebrin loss in dendritic spines occurs at the prodromal stage of Alzheimer's disease (AD), before changes in the density and morphology of dendritic spines. Quantifying drebrin may serve as a potential tool for diagnosing the prodromal stage of AD, before dementia development in AD. PMID: 28865022
- Considering that connexin-43 (Cx43) (along with Cx30) is heavily expressed in astrocytes and that drebrin supports cell-cell contacts, understanding the details of how brain cells live and die reveals molecular pathology involved in neurodegeneration, Alzheimer's disease (AD), other cognitive disorders, and aging. PMID: 28865023
- Further investigations into drebrin and its binding proteins will be crucial for elucidating the pathologies of various diseases and may contribute to the development of medical treatment and diagnostics. PMID: 28865024
- Through its interaction with CXCR4 and the actin cytoskeleton, drebrin regulates T cell activation. CD4(+) T cells are one of the main targets for the human immunodeficiency virus (HIV)-1. PMID: 28865025
- Our research has shown that the immunosuppressant compound BTP2, which blocks Ca(2+) influx into cells, interacts with the actin-reorganizing protein, drebrin. Here we review the role of drebrin in the regulation of calcium signaling, focusing on immune cells. PMID: 28865026
- Upregulation of drebrin has been observed in several types of cancers, e.g., basal cell carcinomas for which it may serve as a marker, liver metastases of colon carcinomas, and bladder cancer, suggesting its involvement in regulating actin dynamics during tumor development, progression, and metastasis. PMID: 28865028
- Drebrin should be included in the list of actin-binding proteins that regulate actin dynamics of mesangial cell processes and foot processes of podocytes. Determining its role in hereditary and acquired glomerulopathies will be crucial. PMID: 28865030
- These findings contribute to the current understanding of cell-cell junction regulation, introducing a new function of drebrin as a stabilizer of endothelial integrity. PMID: 28865031
- Cancer cell motility and invasion are crucial elements in the metastatic cascade and involve dramatic changes in cellular morphology associated with dynamic remodeling of the cytoskeleton. Interestingly, it now appears that drebrin could play this role during cancer development. PMID: 28865033
- Drebrin reduces SMC activation through its interaction with the actin cytoskeleton but independently of its interaction with Homer scaffolds to prevent neointima formation. PMID: 27013612
- Exploring the relationship between drebrin and cognitive function may provide insights into the early prevention of cognitive impairment and in the diagnosis and treatment of Alzheimer's disease. PMID: 26123582
- Drebrin is critical for progranulin-dependent activation of the Akt and MAPK pathways and modulates motility, invasion, and anchorage-independent growth in bladder neoplasms. PMID: 25839164
- The study compared the proteome profiles of the human colon adenocarcinoma cell line HCT-116 with its metastatic derivative E1. DBN1 was found to be overexpressed in E1 compared to HCT-116 cells. PMID: 24610677
- In basal cell carcinoma tissues, intense and homogeneous drebrin expression was observed primarily at tumor cell-cell boundaries. In contrast, drebrin was stained only weakly and non-homogeneously in trichoblastoma and trichoepthelioma tissue samples. PMID: 24346822
- Rab8a and Drebrin E act as key proteins in the regulation of apical trafficking in intestinal epithelial cells. PMID: 24399445
- Drebrin plays a role in HIV infection by modulating viral entry, primarily through the control of actin cytoskeleton polymerization in response to HIV-1. PMID: 23926103
- These findings demonstrate that drebrin contains a cryptic F-actin-bundling activity regulated by phosphorylation and provide a mechanistic model for microtubule-F-actin coupling. PMID: 23979715
- Decreasing the drebrin E levels disrupted the normal subapical F-actin-myosin-IIB-betaII-spectrin network and the apical accumulation of EB3, a microtubule-plus-end-binding protein. PMID: 22275434
- Drebrin contributes to the maintenance of cell shape and may play a significant role in glioma cell motility. PMID: 23201135
- The results of this study showed that hippocampal drebrin loss in mild cognitive impairment. PMID: 22310934
- High expression of cytoskeletal protein drebrin is associated with TEL/AML1pos B-cell precursor acute lymphoblastic leukemia. PMID: 21497902
- Data show that DBN1, SETMAR, and HIG2 are direct transcriptional targets of the SOX11 protein. PMID: 21124928
- The downregulation of Drebrin in Alzheimer's Disease and Down Syndrome and conversely its upregulation in various carcinomas indicate that Drebrin is an important component of the pathogenesis of multiple diseases. PMID: 20183806
- We found decreased levels of drebrin in the frontal and temporal cortex from patients with DS and AD. Reduced drebrin could cause a loss of spine plasticity in DS and may be a marker for neurodegenerative disorders. PMID: 12009525
- These data confirm the role of drebrin E2 in the formation of branching processes and further indicate that during cell migration, the protein contributes to retraction of the cell body and the tail but not to lamellipodia formation. PMID: 16780834
- The down-regulation of drebrin mRNA expression plays a significant role in Alzheimer's disease and is closely related to the progression of the disease. PMID: 18338803
- Drebrin targets EB3 to coordinate F-actin-microtubule interactions that underlie neuritogenesis. PMID: 18806788
Database Links
Subcellular Location
Cytoplasm. Cell projection, dendrite. Cytoplasm, cell cortex. Cell junction. Cell projection, growth cone.
Tissue Specificity
Expressed in the brain, with expression in the molecular layer of the dentate gyrus, stratum pyramidale, and stratum radiatum of the hippocampus (at protein level). Also expressed in the terminal varicosities distributed along dendritic trees of pyramidal
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