DCI1 Antibody
Description
Introduction to DCI1 Antibody
The DCI1 Antibody is a polyclonal antibody targeting dodecenoyl-Coenzyme A delta isomerase (DCI), an enzyme encoded by the DCI gene (NCBI Gene ID: 1632). This antibody is widely used in biomedical research to study DCI's role in metabolic pathways, particularly in fatty acid oxidation and isomerization of 3,2 trans-enoyl-Coenzyme A intermediates .
Key Functional Uses
-
Western Blot: Detects endogenous DCI protein at ~31 kDa in lysates .
-
Immunohistochemistry: Localizes DCI expression in formalin-fixed paraffin-embedded tissues.
-
Immunofluorescence: Visualizes subcellular distribution in cultured cells .
Biological Significance
DCI facilitates the isomerization of unsaturated fatty acyl-CoA derivatives during mitochondrial β-oxidation. Dysregulation of DCI has been implicated in metabolic disorders, though direct disease associations remain under investigation .
Validation and Quality Control
-
Purity: Purified via antigen-affinity chromatography, ensuring minimal cross-reactivity .
-
Batch Consistency: Rigorous validation across species and techniques to ensure reproducibility .
Future Directions
Further studies are needed to explore DCI's role in diseases like cancer or metabolic syndromes. The DCI1 Antibody provides a critical tool for such investigations, particularly in profiling enzyme expression under pathological conditions .
Product Specs
Constituents: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
