DCR2 Antibody
Description
Introduction to DCR2 and Its Antibodies
Decoy receptor 2 (DCR2), also known as TRAIL receptor 4 (TRAILR4) or tumor necrosis factor receptor superfamily member 10D (TNFRSF10D), is a transmembrane receptor that binds tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) but lacks a functional death domain, rendering it incapable of inducing apoptosis. Instead, DCR2 inhibits TRAIL-mediated apoptosis by acting as a decoy receptor . Antibodies targeting DCR2 are critical tools for studying its role in cellular apoptosis, fibrosis, and cancer progression. These reagents enable detection, localization, and functional analysis of DCR2 in diverse biological contexts.
Antibody Types and Applications
DCR2 antibodies are categorized by their clonality, host species, and applications:
Polyclonal Antibodies
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Abcam’s ab4732: A rabbit polyclonal IgG suitable for immunocytochemistry (ICC/IF), western blot (WB), and reacts with Drosophila melanogaster samples. Cited in 13 publications, it detects bands at 171 kDa and 200 kDa, likely due to post-translational modifications .
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Proteintech’s 16781-1-AP: A rabbit polyclonal IgG validated for WB, immunohistochemistry (IHC), and ELISA. It detects human DCR2 at 50–55 kDa and shows reactivity in kidney, prostate, and cancer cell lines .
Monoclonal Antibodies
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Abcam’s EPR3588(2): A rabbit recombinant monoclonal antibody for IHC-P and WB. It reacts with human samples and detects a 35 kDa band, though the predicted molecular weight is 42 kDa .
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Cell Signaling Technology’s D13H4: A rabbit monoclonal IgG for WB and immunoprecipitation (IP). It recognizes endogenous DCR2 in human and monkey samples, with a predicted molecular weight of 45–60 kDa .
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Bio-Techne’s MAB6332: A mouse monoclonal IgG for block/neutralize assays. It targets human TRAILR4 and is azide/BSA-free .
Research Findings and Functional Insights
DCR2 antibodies have elucidated critical roles of DCR2 in apoptosis resistance and fibrosis:
Apoptosis Resistance
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DCR2 overexpression in renal tubular epithelial cells (RTECs) enhances antiapoptotic proteins (FLIP, Bcl2) while suppressing proapoptotic caspases (8 and 3), promoting survival in diabetic nephropathy (DN) .
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DCR2 interacts with GRP78, a chaperone protein, to inhibit caspase-7 activation and activate Akt signaling, thereby sustaining apoptosis resistance .
Fibrosis and Senescence
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DCR2 is upregulated in senescent RTECs and correlates with renal fibrosis markers (α-SMA, collagen IV) in DN models. Its knockdown reduces fibrotic tissue deposition .
Cancer Biology
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DCR2 overexpression confers resistance to TRAIL-induced apoptosis in melanoma and prostate cancer cells, suggesting its role in tumor progression .
Antibody Comparison Table
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Gid8p (Dcr1p) and Dcr2p function in a common pathway to promote START completion in *Saccharomyces cerevisiae*. PMID: 15590836
- Hym1p requires Dcr2p (a phosphatase) and Gid8p (involved in protein degradation) to facilitate the transition from the G1 to S phase of the cell cycle. PMID: 16552603
- Overexpression of DCR2, but not a catalytically inactive DCR2 allele, significantly delays HAC1 splicing and sensitizes cells to the unfolded protein response (UPR). Moreover, Dcr2 physically interacts in vivo with Ire1-S840E,S841E, and dephosphorylates Ire1 in vitro. PMID: 16990850
- Dcr2p phosphatase functionally and physically interacts with Sic1p. Overexpression of Dcr2p destabilizes Sic1p, leading to phenotypes associated with destabilized Sic1p, such as genome instability. PMID: 17673172
KEGG: sce:YLR361C
STRING: 4932.YLR361C
