Dct Antibody
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Soyasaponin Ag inhibits TRP2 expression in a dose-dependent manner, suggesting that its depigmenting effect may be attributed to the inhibition of tyrosinase expression or the enhancement of tyrosinase degradation. PMID: 28713957
- Melanocytes carrying the slaty mutation exhibit heightened sensitivity to ultraviolet rays and oxidative stress compared to melanocytes responsible for black pigmentation. PMID: 26451690
- A novel murine model for vitiligo has been established through sequential prime/boost immunizations using the tyrosinase-related protein 2 (TRP2)-180 (SVYDFFVWL) peptide in the hind footpad and tail dermis. PMID: 23711243
- Studies indicate that the tumor antigen TRP2, expressed in murine cytomegalovirus (MCMV), induces a robust and enduring anti-melanoma effect through an antibody-dependent mechanism. PMID: 23811402
- Vaccine formulations employing synthetic long peptides for treating B16F10 skin melanoma have failed to elicit strong CD8 T cell responses against self-differentiation antigens TRP-2 and gp100. PMID: 23203930
- The level of gene targeting was not enhanced by the induction of double-strand breaks, suggesting a limited capacity of I-SceI to facilitate homologous recombination at the Dct locus. PMID: 22761925
- Recombinant dopachrome tautomerase has been successfully expressed and purified, excluding its carboxy-terminal transmembrane region. Analysis of dopachrome tautomerase tryptic peptides using MALDI-TOF/TOF identified N-glycosylation as a primary post-translational modification. PMID: 20386969
- Inactivation of dopachrome tautomerase elevates reactive oxygen species (ROS) levels, increases the number of sunburn cells and apoptotic cells, and reduces eumelanin content in the epidermis following chronic UVA radiation exposure. PMID: 20123016
- The TRP-2 polypeptide undergoes folding within the endoplasmic reticulum (ER) in the presence of calnexin, until it attains a dithiothreitol-resistant conformation, enabling its exit from the ER to the Golgi. PMID: 12719423
- Results describe the generation of a dopachrome tautomerase gene knockout in mice, with effects confined to pigment production and coat color. PMID: 15060160
- It has been observed that the ionophore monensin (Mon) and the quaternary amine chloroquine (CQ) discriminate between the trafficking routes of TRP-2 and TRP-1. PMID: 15707965
- The enzymatic activity of Dct may play a role in determining the preference for either the eumelanin or pheomelanin pathway during pigment biosynthesis. PMID: 15960609
- Findings suggest that the slaty mutation influences both eumelanin and pheomelanin synthesis in a developmental stage-specific and skin site-specific manner. PMID: 16584806
- The slaty mutation disrupts melanosome maturation at stage III and affects melanosome morphology (elliptical or spherical) in a developmental stage-dependent manner. PMID: 17516460
- Eumelanin and pheomelanin may accumulate with difficulty in slaty-mutated melanocytes and are easily released during skin development. L-Tyr may stimulate this release. PMID: 17551240
- These findings suggest that the slaty mutation inhibits the development of elliptical stage IV melanosomes, while L-Tyr restores the development of these melanosomes. PMID: 17867832
- Cytotoxicity toward targets loaded with a K(b)-restricted tyrosinase-related protein 2-derived peptide was correlated with depigmentation in a mouse model of autoimmune vitiligo. PMID: 18337834
- Mice lacking DCT exhibited normal cardiac development but an increased susceptibility to atrial arrhythmias. PMID: 19855129
Q&A
Basic Research Questions
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What is DCT antibody and what are its principal research applications?
DCT (Dopachrome tautomerase) is also known as TYRP2, TRP-2, L-dopachrome tautomerase, and L-dopachrome Delta-isomerase. It is a protein with a calculated molecular weight of approximately 59 kDa that plays a role in melanin synthesis pathways .
DCT antibodies are extensively utilized across multiple experimental techniques in research settings. Based on published applications, these antibodies have been successfully employed in:
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Western Blot (WB): For protein detection and quantification in cell and tissue lysates
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Immunohistochemistry (IHC): For localization studies in tissue sections
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Immunofluorescence (IF/ICC): For subcellular localization in cultured cells
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Immunoprecipitation (IP): For protein isolation and interaction studies
When designing experiments, researchers should select antibodies with validation data for their specific application and target species to ensure reliable results.
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What are the recommended dilutions for DCT antibody across different experimental applications?
Optimal antibody dilution is critical for achieving specific signal while minimizing background. For DCT antibody, the following dilution ranges have been empirically determined and validated:
Application Recommended Dilution Western Blot (WB) 1:500-1:2000 Immunoprecipitation (IP) 0.5-4.0 μg for 1.0-3.0 mg total protein lysate Immunohistochemistry (IHC) 1:20-1:200 Immunofluorescence (IF/ICC) 1:200-1:800 These ranges provide starting points, but researchers should note that optimal dilutions are sample-dependent and may require titration in each specific experimental system . When troubleshooting, perform a dilution series to determine the optimal concentration that maximizes signal-to-noise ratio for your particular samples.
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What species reactivity does DCT antibody demonstrate and how is cross-reactivity validated?
The DCT antibodies described in the search results demonstrate reactivity with multiple species. According to available data, tested reactivity includes human, mouse, rat, and monkey samples . Some commercially available antibodies may also react with additional species including rabbit, bovine, dog, guinea pig, hamster, pig, and sheep, though these require independent validation .
Researchers should verify species cross-reactivity through:
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Positive control samples from target species
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Sequence homology analysis between species
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Western blot confirmation using target species lysates
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Tissue sections with known DCT expression patterns
When working with species not listed in the manufacturer's validated reactivity panel, preliminary validation experiments are essential to confirm antibody specificity before proceeding with full studies.
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How should DCT antibody be stored and handled for optimal performance and longevity?
Proper storage and handling of DCT antibody is crucial for maintaining its activity and specificity. Based on manufacturer recommendations, DCT antibodies should be:
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Stored at -20°C for long-term stability
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Stored in buffer containing PBS with 0.02% sodium azide and 50% glycerol at pH 7.3
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Considered stable for one year after shipment when properly stored
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Aliquoted to avoid repeated freeze-thaw cycles when using larger volumes (optional for -20°C storage)
When working with the antibody, researchers should:
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Thaw aliquots completely before use and mix gently
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Avoid prolonged exposure to room temperature
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Return to -20°C promptly after use
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Maintain sterile technique when handling to prevent microbial contamination
Following these protocols will help maintain antibody integrity throughout the course of a research project.
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What positive controls are recommended for validating DCT antibody performance?
Selection of appropriate positive controls is essential for confirming antibody specificity and optimizing experimental conditions. For DCT antibody, the following positive controls have been empirically validated:
For Western blot applications:
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HL-60 cells
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A375 cells (melanoma cell line with high DCT expression)
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A431 cells
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COS-7 cells
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MCF-7 cells
For immunoprecipitation:
For immunohistochemistry:
For immunofluorescence:
When establishing a new experimental system, these validated controls provide reliable reference points. Additionally, melanocyte-derived cell lines or tissues with melanocyte populations are generally suitable positive controls for DCT expression studies.
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