dnc-6 Antibody
Description
Target Background
4,4'-dinitrocarbanilide (DNC) is a metabolite of nicarbazin, a veterinary antiparasitic drug. Its detection is critical for food safety monitoring due to residue concerns in animal products .
Antibody Development
Hybridoma Technology:
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Fusion rate: ~80%
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Positive hybridoma rate: ~15%
-
Final stable cell lines: 4 clones (2B5-F3-C12, 2B5-G1-G7, 2B5-C3-G1, 2B5-F3-E8)
| Hybridoma Clone | Ascites Antibody Titer | Stability Post-Cryopreservation |
|---|---|---|
| 2B5-G1-G7 | 160,000 | Stable for ≥1 month |
| Others | >10,000 | Stable for ≥1 month |
Functional Characteristics
Target Profile
Claudin 6 (CLDN6) is a tight junction protein overexpressed in ovarian (29%), endometrial (11%), and other cancers, with minimal normal tissue expression .
2.2.1 Specificity Mechanisms
| Feature | IM171/IM301/IM302 MAbs | Conventional CLDN6 MAbs |
|---|---|---|
| Cross-reactivity | None with CLDN9/CLDN3/CLDN4 | Frequent cross-reactivity |
| Structural basis | Steric hindrance at Q156γ-carbon | Direct epitope binding |
| Affinity | ≤1 pM | Variable (often lower) |
| Epitope conservation | Binds conserved residues | Targets variable regions |
2.2.2 Preclinical Efficacy (CLDN6–23-ADC)
| Parameter | Result |
|---|---|
| Binding affinity (KD) | 2.32 nM (KinExA) |
| In vitro EC50 | 15.96 nM (OVCA-429 cells) |
| Tumor regression rate | 100% (UMUC4 xenografts) |
| Survival benefit | >100 days (PDX models) |
Clinical Relevance
Critical Terminology Analysis
The term "dnc-6" likely stems from one of these scenarios:
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Typographical error: "dnc" instead of "CLDN" (claudin abbreviation)
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Nomenclature confusion: Merging "DNC" (chemical) with "CLDN6" (biological target)
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Undocumented antibody: No peer-reviewed publications match "dnc-6 Antibody" terminology.
Research Recommendations
Product Specs
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
