DDIT4L Antibody
Description
Introduction to DDIT4L and Its Antibody
DDIT4L is a protein encoded by the DDIT4L gene (OMIM 607730), which regulates cellular metabolism and stress responses by inhibiting mTOR signaling upstream of the TSC1-TSC2 complex . The DDIT4L antibody is designed to detect this protein in various experimental contexts, enabling researchers to study its localization, expression levels, and functional roles in diseases such as cancer, cardiomyopathy, and autoimmune disorders .
Applications of DDIT4L Antibody
The antibody is validated for multiple techniques:
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Western Blot (WB): Detects endogenous DDIT4L in tissues (e.g., mouse ovary) and cell lines .
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Immunohistochemistry (IHC): Used to analyze protein expression in human tissues .
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Immunoprecipitation (IP): Facilitates studies of protein-protein interactions, such as its binding to mTOR .
Research Findings and Utilization
The antibody has been instrumental in studies linking DDIT4L to:
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Autophagy Regulation: Confirmed DDIT4L's role in inhibiting mTORC1 signaling and promoting stress-induced autophagy in cardiomyocytes .
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Immune Modulation: Demonstrated DDIT4L's suppression of Th17 cell differentiation via the DDIT4/mTOR pathway in multiple sclerosis models .
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Mitochondrial Dynamics: Showed DDIT4L reduces mitochondrial mass and respiratory capacity in neonatal myeloid cells .
Example Citation:
"Using a DDIT4L antibody (PA5-100240), researchers observed increased autophagy markers (LC3-II) in cardiac tissues with pathological stress ."
Product Specs
Target Background
- DDIT4L mediates monocyte cell death by reducing thioredoxin-1 expression. PMID: 19268525
- Promoter methylation and differential gene expression of five markers (COL1A2, NPM2, HSPB6, DDIT4L, and MT1G) were validated through sequencing of bisulfite-modified DNA and real-time reverse transcriptase PCR, respectively. PMID: 19491193
