DDX19B Antibody
Description
Introduction
The DDX19B antibody is a polyclonal or monoclonal reagent designed to detect and study the DEAD-box RNA helicase DDX19B protein. DDX19B is a key regulator of mRNA export, translation termination, and nonsense-mediated mRNA decay (NMD). Its role in cellular processes such as nuclear export of viral RNAs and interaction with translation initiation factors has made it a focal point in molecular biology and virology research.
The antibody is primarily used in Western blot (WB), immunoprecipitation (IP), and immunocytochemistry (ICC) applications. This article synthesizes data from diverse sources to provide a detailed analysis of its technical specifications, research applications, and key findings.
2.1. Antibody Characteristics
2.2. Recommended Dilutions
| Application | Dilution |
|---|---|
| Western Blot | 1:500–1:3000 |
| Immunoprecipitation | 0.5–4.0 µg/1–3 mg lysate |
| ELISA | Varied (optimize per assay) |
3.1. mRNA Export and Viral Pathogenesis
The DDX19B antibody has been instrumental in studying its role in mRNA export. For instance, in influenza A virus (IAV) replication, DDX19B facilitates the nuclear export of viral RNAs, as shown by its depletion leading to delayed viral protein synthesis . Rescue experiments with RNA-binding defective mutants (e.g., R372G, R428Q) confirmed that RNA-binding activity is critical for this process .
3.2. Translation Regulation
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CTIF Interaction: DDX19B binds CTIF (CBC-dependent translation initiation factor) to restrict translation initiation to the perinuclear region. Disruption of this interaction via mutations (e.g., F460A) causes uncontrolled cytoplasmic translation and NMD dysregulation .
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Translation Termination: DDX19B stabilizes ribosomal complexes with eRF1 (eukaryotic release factor 1), enhancing peptide release during termination .
3.3. Nonsense-Mediated Decay (NMD)
DDX19B inhibits NMD by modulating CTIF activity on CBC-bound mRNAs. Overexpression of CTIF mutants lacking DDX19B binding (e.g., F460A) increases NMD efficiency, as observed in globin and GPx1 reporter assays .
4.1. DDX19B in Cellular Pathways
4.2. Viral Interactions
Product Specs
Target Background
- These findings suggest that DBP5, GLE1, and IP6 have distinct and conserved roles in cytoplasmic mRNA expression. Variations in phenotype arise from the specific functions of each protein in intracellular mRNA metabolism. PMID: 29746542
- DDX19 interacts with pre-termination complexes (preTCs) in a nucleotide-dependent manner. Moreover, DDX19 enhances the efficiency of termination complex (TC) formation and peptide release in the presence of eukaryotic release factors. DDX19 also stabilizes ribosome complexes with translation elongation factors eEF1 and eEF2. PMID: 28180304
- Ddx19 is an RNA export factor essential for nuclear import of the SRF coactivator MKL1. PMID: 25585691
- Dbp5, Gle1-IP6, and Nup159: a working model for mRNP export. PMID: 22064466
- Identification and characterization of a gene encoding a novel isoform. PMID: 12219940
- In vitro assays demonstrate that NUP214 reduces both the RNA binding and ATPase activities of DBP5. PMID: 19219046
- Results describe the crystal structures of human DDX19, a DEXD/H-box protein, in its open and closed cleft conformations. PMID: 19244245
