DED1 Antibody
Description
Target Protein Overview
Ded1 is an essential RNA helicase involved in:
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Unwinding 5’-UTR secondary structures to facilitate ribosomal scanning
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48S preinitiation complex (PIC) assembly during translation initiation
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Translation repression under TORC1 inhibition and stress conditions
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Functional interplay with paralog Dbp1 to regulate mRNA-specific translation
Structurally, Ded1 contains:
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Central helicase core with ATPase motifs
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N- and C-terminal intrinsically disordered regions (IDRs) mediating protein interactions
Protein Localization Studies
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Identified endoplasmic reticulum-associated foci under stress using GFP-tagged Ded1
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Detected stress granule (SG) accumulation in ATPase-deficient mutants (ded1-E307A, ded1-R489A)
Functional Analyses
Regulatory Roles in Translation
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Dual functionality: Ded1 promotes translation initiation in nutrient-rich conditions but represses bulk translation during TORC1 inhibition .
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Structural dependence: mRNAs with long (>100 nt), structured 5’-UTRs exhibit 2–4-fold greater Ded1 dependency .
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Paralog compensation: Dbp1 overexpression rescues translation defects in ded1-ts mutants for 44% of Ded1-hyperdependent mRNAs .
Stress Response Mechanisms
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Oxidative stress: Ded1-ΔCT mutants show 53% reduced viability post-H₂O₂ treatment and delayed polysome recovery .
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Thermal adaptation: ded1-cs mutants display cold-sensitive growth defects linked to impaired 43S PIC scanning .
Technical Considerations
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Epitope tagging: Internal 3V5 tags (e.g., inserted between residues 213–214) preserve Ded1 function better than C-terminal tags .
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Cross-reactivity: No reported cross-reactivity with Dbp1 due to low sequence identity (27%) .
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Quantitative limitations: Dbp1 protein levels are 3-fold lower than Ded1 despite comparable mRNA levels, necessitating sensitive detection methods .
Unresolved Questions
Product Specs
Components: 50% Glycerol, 0.01M PBS, pH 7.4
