Defensin D2 Antibody, Biotin conjugated
Description
Product Overview
The Defensin D2 Antibody, Biotin conjugated, is a polyclonal antibody raised in rabbits against recombinant Spinacia oleracea (spinach) Defensin D2 protein (1-52 amino acids). It is designed for immunoassays such as ELISA and Western blotting, with demonstrated reactivity in Spinacia oleracea samples . Key product specifications include:
| Parameter | Details |
|---|---|
| Antibody Type | Polyclonal Rabbit IgG |
| Conjugation | Biotin |
| Immunogen | Recombinant Spinacia oleracea D2 |
| Reactivity | Spinacia oleracea |
| Applications | ELISA, Western blot (untested) |
| Storage | -20°C (avoid freeze-thaw cycles) |
Research Applications
Defensin D2 Antibody has been utilized in studies investigating antimicrobial peptides (AMPs) and their mechanisms of action. For example:
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Antimicrobial Activity: Defensin D2 exhibits broad-spectrum efficacy against Fusarium spp., Gram-positive (e.g., Staphylococcus), and Gram-negative bacteria (e.g., Pseudomonas), as demonstrated in proteomic studies .
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Immune Signaling: Defensins activate neutrophil receptors (e.g., Mrgpra2), triggering cytokine release (IL-1β, Cxcl2) and immune response amplification .
Related Antibodies and Cross-Reactivity
Similar biotin-conjugated antibodies targeting human beta-defensins (e.g., BD-2) exist, such as:
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ARG56742 (Goat Polyclonal, Human): Tested for ELISA and Western blot, with specificity for E. coli-derived recombinant BD-2 .
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BAF2758 (R&D Systems): Validated for Western blot in human samples, targeting beta-defensin 4/2 .
Technical Considerations
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Biotinylation Methods: NHS-biotin conjugation may alter antibody specificity, as observed in defensin-targeting monoclonals . Glycan-linked biotinylation preserves specificity .
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Storage and Handling: Antibodies should be stored at -20°C to prevent degradation. Avoid repeated freeze-thaw cycles .
Research Findings
Product Specs
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
