del-1 Antibody

Overview of DEL-1 Antibody

DEL-1 (EDIL3) is an extracellular matrix protein expressed by endothelial cells and macrophages. It regulates leukocyte recruitment, efferocytosis (apoptotic cell clearance), and myelopoiesis through interactions with integrins (e.g., αvβ3, β2 integrins) and phosphatidylserine (PS) . Antibodies targeting DEL-1 are critical for studying its role in inflammatory diseases, such as arthritis, multiple sclerosis, and vascular disorders.

Key Features of DEL-1 Antibodies

DEL-1 antibodies vary in clonality, host species, and validated applications. Below is a comparative analysis of commercially available antibodies:

Notes:

• NBP1-28632 is BSA-free and validated for immunocytochemistry at 1:100 dilution .

• ab190692 is suitable for intracellular flow cytometry and immunoprecipitation .

• AF6046 detects EDIL3 in neuroblastoma (SH-SY5Y) and breast cancer (MDA-MB-231) cell lines .

Applications in Research

DEL-1 antibodies are employed in diverse techniques to study protein expression and function:

Western Blot (WB)

• NBP1-28632: Detects a ~53 kDa band in human brain lysate (WB) and ~61 kDa in Simple Western (Size-Wes) .

• ab190692: Validates a 54 kDa band in 293, C6, RAW 264.7, and NIH/3T3 cell lysates .

• AF6046: Identifies 52–54 kDa bands in SH-SY5Y, MDA-MB-231, HFL1, and MRC-5 cell lysates .

Immunocytochemistry (ICC)/Immunofluorescence (IF)

• NBP1-28632: Labels endothelial and immune cells at 1:100 dilution .

• AF6046: Stains cytoplasmic EDIL3 in MDA-MB-231 cells (red fluorescence) .

Immunoprecipitation (IP)

• ab190692: Efficiently pulls down EDIL3 from HeLa cell lysates .

Flow Cytometry

• ab190692: Detects intracellular EDIL3 in fixed HeLa cells .

Molecular Weight Variability

The observed molecular weight (MW) of DEL-1 varies due to post-translational modifications:

• NBP1-28632: Theoretical MW = 53 kDa; observed = 61 kDa in Simple Western .

• AF6046: Observed MW = 52–54 kDa, aligning with the theoretical 53–54 kDa .

Contextual Relevance in Disease Models

While DEL-1 antibodies themselves are not used therapeutically, studies using Del1 knockout mice highlight the protein’s role in:

• Arthritis: Endothelial DEL-1 overexpression reduces arthritis severity by limiting leukocyte recruitment and inhibiting Tfh cell differentiation .

• Inflammation Resolution: Macrophage-derived DEL-1 promotes efferocytosis and resolution-phase macrophage reprogramming .