At2g36255 Antibody

Target Protein Characteristics

The At2g36255 gene encodes a Defensin-like (DEFL) family protein (UniProt ID: Q2V424), part of a conserved group of cysteine-rich polypeptides involved in plant innate immunity . Key features:

Experimental Applications

The antibody's primary uses include:

• Western blot analysis: Detecting native DEFL proteins in A. thaliana extracts

• ELISA-based quantification: Measuring protein expression levels under different stress conditions

• Immunolocalization studies: Mapping protein distribution in plant tissues (pending validation)

Recent protocols suggest optimal performance when using 1:500-1:2000 dilution in WB applications, though exact conditions require empirical optimization .

Research Context

While direct studies using this specific antibody remain limited in public databases, defensin-like proteins in Arabidopsis are known to:

• Participate in pathogen response pathways

• Regulate root development

• Influence symbiotic relationships with soil microbes

The antibody enables investigation of these processes through protein-level analyses complementary to genetic studies.

Validation Status

Current validation data indicates:

• Clear band detection at ~15 kDa (predicted molecular weight) in Western blots

• Specificity confirmed through recombinant protein controls

• No cross-reactivity reported with other DEFL family members in initial tests

Technical Considerations

Users should note:

• Requires antigen retrieval methods for formalin-fixed samples

• May show reduced affinity to post-translationally modified forms

• Optimal results obtained with fresh plant extracts rather than frozen stocks