At5g18403 Antibody

Contextual Analysis of AT5G18403

Gene details:

• Locus: AT5G18403

• Chromosomal location: Chromosome 5 (nucleotide 6,114,963 to 6,116,032)

• Protein: Uncharacterized DUF966 family protein (268 amino acids)

• Expression: Limited public data; weakly expressed in roots and siliques based on Arabidopsis eFP Browser.

Functional insights:

• DUF966 domain: A conserved domain of unknown function, prevalent in plants.

• Homologs: Found in Oryza sativa (rice) and Zea mays (maize), but functional studies are absent.

Antibody Development Challenges for Uncharacterized Proteins

While no AT5G18403-specific antibody is documented, general principles for plant protein antibody development include:

Related Antibody Workflows in Plant Research

Examples from the search results highlight methodologies that could apply to AT5G18403:

A. Recombinant Antibody Production ( ):

• Phage display or hybridoma technology for monoclonal antibody generation.

• Requires cloning AT5G18403 into expression vectors (e.g., E. coli, CHO cells).

B. Validation Protocols ( ):

• Western blot: Compare wild-type vs. CRISPR-edited Arabidopsis lines.

• Immunoprecipitation-MS: Identify interacting partners.

C. Commercial Antibody Standards ( ):

• Polyclonal antibodies (e.g., ATG5 Antibody in ) use peptide immunogens (1-30 aa regions).

• Storage: -20°C in aliquots to prevent freeze-thaw cycles.

Research Gaps and Recommendations

1. Gene Characterization: Prioritize functional studies (e.g., knockout phenotyping, subcellular localization).

2. Antigen Design: Use in silico tools (e.g., I-TASSER, AlphaFold) to predict immunogenic regions.

3. Collaborative Efforts: Partner with repositories (e.g., Arabidopsis Biological Resource Center) for gene-specific reagents.

References to Methodological Frameworks

• Recombinant antibody production ( ): GENEWIZ workflows for codon-optimized synthesis.

• Antibody validation ( ): YCharOS standards using KO cell lines and orthogonal assays.

• Glycan-targeting antibodies ( ): Structural insights for plant-specific epitopes.