At5g50423 Antibody

Functional Role of At5g50423 Protein

The At5g50423 gene encodes a defensin-like protein implicated in:

• Membrane-associated processes: Predicted roles in lipid binding or transport due to its classification as a putative membrane lipoprotein .

• Plant defense mechanisms: Defensin-like proteins often exhibit antimicrobial activity, suggesting a role in pathogen response .

• Developmental regulation: Indirect associations with cell wall integrity and secretion pathways, based on homolog studies (e.g., AtSYP32 in pollen wall development) .

Experimental Use Cases

• Protein Localization: Used to confirm cytoplasmic expression in Arabidopsis tissues via Western blot and IHC .

• Mutant Phenotyping: Employed to assess protein abundance in atsyp32+/− mutants, revealing disrupted secretory pathways and pollen wall defects .

• Interaction Studies: Potential utility in mapping protein-protein interactions, though specific partners remain uncharacterized .

Key Data from Studies

Limitations and Future Directions

• Specificity Gaps: Limited validation in non-Arabidopsis species or complex plant tissues .

• Functional Insights: No direct mechanistic studies linking At5g50423 to defense or developmental pathways exist yet .

• Therapeutic Potential: Unlike monoclonal antibodies in clinical use (e.g., LY-CoV555 for COVID-19 ), plant-focused antibodies like At5g50423 remain research tools .