At5g60805 Antibody

Biological Context

The At5g60805 gene is located on chromosome 5 of Arabidopsis thaliana. While its specific biological role hasn't been fully elucidated, antibodies against such plant proteins typically enable researchers to:

• Map protein expression patterns via immunohistochemistry

• Verify gene editing outcomes through Western blot analysis

• Study protein-protein interactions in developmental processes

Current antibody validation standards emphasize the importance of:

1. Target-specific binding confirmation using knockout controls

2. Application-specific performance testing (e.g., Western Blot vs. immunofluorescence)

3. Batch-to-batch reproducibility documentation

Research Applications

Though no direct studies using At5g60805 Antibody were identified in the reviewed literature, comparable plant antibody applications include:

Synthesis from antibody characterization principles

Characterization Status

Available data indicates this antibody requires further experimental validation:

• No peer-reviewed publications specifically referencing CSB-PA648850XA01DOA were identified

• Standard validation metrics (e.g., KO cell line testing) not publicly documented

• Antigen immunogen details and epitope mapping unavailable in cited sources

This reflects a broader challenge in plant antibody research, where approximately 50-75% of commercial reagents lack sufficient characterization data according to recent meta-analyses .

Technical Considerations

For experimental use with this antibody:

• Recommended initial dilution: 1:500-1:2000 (based on comparable products)

• Suggested controls:

  • Arabidopsis wild-type vs. At5g60805 knockout lines

  • Pre-immune serum controls

  • Secondary antibody-only conditions

The antibody's performance may vary significantly between applications, emphasizing the need for application-specific optimization .