At3g45093 Antibody

Definition and Target Characterization

The At3g45093 antibody is a polyclonal antibody raised against the protein product of the At3g45093 locus in Arabidopsis thaliana (Mouse-ear cress). This gene’s protein is annotated under UniProt accession Q2V3Q8, though its precise biological function remains under investigation. The antibody is primarily used to detect and study the expression, localization, and interactions of this protein in plant research models .

Research Applications and Protocols

While specific protocols for this antibody are not detailed in publicly accessible literature, standard methodologies for plant protein analysis apply:

• Western Blot: Typically used at dilutions between 1:500–1:2000, depending on sample type and detection system .

• Immunolocalization: For subcellular localization studies in Arabidopsis tissues, concentrations of 2–5 µg/mL are common .

• Cross-Reactivity: No cross-reactivity with non-Arabidopsis species has been reported, though validation in related plant models (e.g., Brassica) is advised .

Scientific Context and Unresolved Questions

The At3g45093 gene is part of a larger family of uncharacterized genes in Arabidopsis. Insights from analogous antibodies (e.g., TBX3 in humans , SCRL proteins in plants ) suggest potential roles in developmental regulation or stress responses. Key research gaps include:

• Functional Role: No knockout or overexpression studies for At3g45093 are documented.

• Protein Interactions: No known binding partners or pathways have been identified.

• Expression Patterns: Spatial/temporal expression data in Arabidopsis organs or under stress conditions are lacking.

Comparative Insights from Related Antibodies

Studies on antibodies targeting similar plant proteins (e.g., SCRL family , DRM2 ) highlight the importance of rigorous validation:

• Epitope Stability: Plant-specific post-translational modifications may affect antibody binding .

• Technical Challenges: High background noise in plant lysates necessitates optimized blocking buffers (e.g., 5% non-fat milk in TBST) .

Future Directions

• Functional Studies: CRISPR/Cas9-generated mutants could elucidate At3g45093’s role.

• Omics Integration: Proteomic or transcriptomic datasets (e.g., TAIR) may reveal co-expressed genes.

• Therapeutic Potential: While plant antibodies are not directly used in human therapy, insights into conserved domains could inform engineering of bispecific antibodies .