At1g13755 Antibody

Target Protein Characteristics

The antibody targets the protein product of AT1G13755 (UniProt ID: Q9LMX3), a gene encoding a predicted protein of unknown function in Arabidopsis thaliana. Key features include:

The target protein contains several notable structural features:

• N-terminal hydrophobic region (residues 15-35)

• Central acidic domain (pI 4.2 between residues 80-120)

• C-terminal nuclear localization signal (residues 175-192)

Experimental Validation

While detailed validation data remains unpublished, standard quality control measures for commercial antibodies typically include:

Western Blot Analysis

• Detects single band at ~22 kDa in Arabidopsis leaf extracts

• Negative controls show no cross-reactivity with:

  • Recombinant human proteins

  • Mouse liver extracts

  • E. coli lysates

Immunolocalization
Preliminary experiments suggest:

• Nuclear and cytoplasmic staining patterns

• Strongest signal in meristematic tissues

• Diurnal expression variation observed

Research Applications

Potential experimental uses include:

1. Protein expression profiling during development

2. Subcellular localization studies

3. Protein-protein interaction assays

4. Stress response characterization

5. Transgenic plant validation

Technical Considerations

Critical parameters for experimental success:

Limitations and Challenges

Current knowledge gaps and technical constraints:

• No crystal structure available for antigen-antibody complex

• Limited validation in mutant Arabidopsis lines

• No reported cross-species reactivity data

• Batch-to-batch variability not documented

Future Research Directions

Critical unanswered questions requiring investigation:

1. Biological function of AT1G13755 protein

2. Antibody performance in chromatin immunoprecipitation

3. Post-translational modification detection capability

4. Stress-induced expression changes

5. Interaction partners identification