At2g04033 Antibody

Molecular Identification of At2g04033 Antibody

At2g04033 antibody is a rabbit-derived polyclonal antibody designed to detect the protein encoded by locus At2g04033 in Arabidopsis thaliana (Mouse-ear cress). This antibody targets the protein product of the gene annotated under UniProt accession P0CAY8, though the exact biological function of this protein remains uncharacterized in publicly available literature .

Key attributes:

• Host species: Rabbit

• Immunogen: Full-length recombinant At2g04033 protein (specific immunogen sequence undisclosed)

• Reactivity: Specific to Arabidopsis thaliana with potential cross-reactivity to orthologs in related Brassicaceae species

Research Applications

Validated experimental uses include:

• Western Blot: Confirmed detection of recombinant At2g04033 protein at expected molecular weight

• ELISA: Quantitative analysis with high sensitivity (picogram-level detection)

• Immunoprecipitation: Presumed capability based on polyclonal nature, though unpublished verification data

Notably, this antibody has not been reported in peer-reviewed studies for advanced applications such as:

• Chromatin immunoprecipitation (ChIP)

• Immunofluorescence microscopy

• Plant tissue-specific expression profiling

Quality Control Metrics

Manufacturers provide batch-specific validation:

• Purity: Protein A/G affinity-purified (>95% by SDS-PAGE)

• Stability: 12 months at -20°C with repeated freeze-thaw cycles discouraged

• Specificity: Verified against preimmune serum controls showing no background reactivity

Limitations and Considerations

1. Epitope Mapping: Exact binding regions undefined, limiting epitope-specific studies

2. Plant Developmental Staging: No published data on antibody performance across growth stages

3. Post-Translational Modifications: Cross-reactivity with phosphorylated/glycosylated forms unverified