At2g25185 Antibody

Definition and Target

The At2g25185 Antibody (Product Code: CSB-PA651202XA01DOA) is a polyclonal antibody developed to detect the protein encoded by the At2g25185 gene in Arabidopsis thaliana (Mouse-ear cress). This gene locus corresponds to UniProt accession Q2V459, though the functional role of the protein remains uncharacterized in publicly available literature .

Antibody Utility in Plant Studies

• Antibodies like At2g25185 are critical for studying gene expression, protein localization, and post-translational modifications in model plants .

• Arabidopsis thaliana is a widely used organism in plant genetics, making this antibody relevant for comparative studies of gene families or stress-response pathways.

Comparative Analysis

The At2g25185 Antibody is part of a broader catalog of Arabidopsis-specific reagents. For example:

• LCR21 Antibody (Code: CSB-PA306806XA01DOA) targets a different locus (UniProt: P82735) involved in root development .

• DDM1 Antibody (Code: CSB-PA894029XA01DOA) detects a chromatin-remodeling protein with well-documented roles in DNA methylation .

Unlike these better-characterized targets, At2g25185’s functional annotation remains incomplete, suggesting it may represent a novel or understudied protein.

Recommendations for Use

1. Validation Steps:

   • Perform Western blotting on Arabidopsis wild-type vs. At2g25185 knockout lines to confirm specificity.

   • Use secondary antibodies with minimal cross-reactivity (e.g., HRP-conjugated anti-rabbit IgG) .

2. Applications:

   • Subcellular localization via immunofluorescence.

   • Protein interaction studies using co-immunoprecipitation.

Limitations and Gaps

• No peer-reviewed studies directly utilizing this antibody were identified, limiting insight into its performance.

• The UniProt entry Q2V459 lacks functional annotations, hindering biological interpretation of results.