DGKQ Antibody
Description
Tested Applications
Validated uses across tissues and cell lines include:
Western Blot (WB)
Immunohistochemistry (IHC)
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Positive staining in human colon cancer, cervix, and mouse brain tissues .
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Antigen retrieval with TE buffer (pH 9.0) or citrate buffer (pH 6.0) .
Immunofluorescence (IF-P)
Research Applications
DGKQ antibodies facilitate studies on DGKQ’s role in cellular signaling and disease:
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Immune Regulation: DGK isoforms (α, ζ, θ) limit diacylglycerol (DAG)-mediated T cell activation. DGK inhibition enhances cytokine production (e.g., IL-2, IFNγ) and anti-tumor responses .
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Cancer Research: DGKQ is implicated in renal cell carcinoma and glioblastoma. Antibodies enable tracking DGKQ expression in tumor microenvironments .
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Signal Transduction: DGKQ interacts with RHOA, influencing nuclear phosphatidylinositol cycling .
Key Research Findings
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T Cell Activation: DGKQ modulates DAG levels, affecting downstream effectors like RasGRP1 and PKCθ .
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Therapeutic Potential: DGK inhibitors (e.g., R59022) reverse NK cell dysfunction in renal carcinoma .
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Structural Insights: DGKQ contains a pleckstrin homology domain critical for nuclear speckle localization .
Product Specs
Target Background
- Research demonstrates that DGKθ plays a significant role in steroid hormone production within human adrenocortical cells. PMID: 24369117
- The transcription factors SF1 and SREBP1 activate DGKθ expression in a cAMP-dependent manner in human adrenocortical cells. PMID: 23610160
- Studies have revealed that the minor alleles of GAK rs1564282 and DGKQ rs11248060 are associated with an increased risk of Parkinson's disease in Han Chinese populations. PMID: 23618683
- Evidence suggests that DGKθ is a key mediator of bile-acid-stimulated modulation of the farnesoid X receptor/mTOR complex 2/Akt signaling pathway, influencing glucose metabolism and homeostasis in hepatocytes. PMID: 23767959
- The translocation and activity of DGKθ are regulated by the combined actions of the epidermal growth factor receptor (EGFR) and protein kinase C (PKC). PMID: 22732145
- DGKθ directly interacts with and is activated by basic proteins such as histone H1 and Tau with nanomolar affinity, suggesting a potential role for a polybasic protein or protein domain in the activation of this enzyme. PMID: 23091060
- Nuclear DGKθ co-localizes with phosphatidylinositol 4,5-bisphosphate (PIP(2)) in domains that correspond to nuclear speckles. PMID: 12799190
- DGK interacts selectively with and is phosphorylated by PKCε and -η. Peptide agonist-induced selective activation of PKCε directly leads to DGK translocation. PMID: 15632189
